基于肠道菌群和代谢组学探讨复方蜘蛛香凝胶贴膏治疗消化不良的作用机制OA

Objective:To explore the mechanism of Compound Zhizhuxiang(蜘蛛香)Gel Plaster in the treatment of dyspepsia based on gut microbiota and metabolomics techniques.Methods:A high-calorie-diet-induced dyspepsia(HC-DID)model was established in young mice.The therapeutic effect of Compound Zhizhuxiang Gel Plaster on dys-pepsia was evaluated based on changes in body weight and food intake,gastric emptying rate,small intestinal propulsion rate,and pathological changes in the gastric antrum and duodenum.ELISA was used to detect the serum levels of D-xy-lose,cholecystokinin(CCK),vasoactive intestinal peptide(VIP),5-hydroxytryptamine(5-HT),and motilin(MTL).Changes in gut microbiota and metabolites in intestinal contents were analyzed by 16S rDNA high-throughput sequencing and ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS).Results:Compared with the normal control group,the model control group showed significantly reduced food intake and body weight,as well as suppressed gastric emptying rate and small intestinal propulsion rate(P<0.05).Compared with the model control group,Compound Zhizhuxiang Gel Plaster significantly increased food intake and body weight,enhanced gastric emptying and small intestinal propulsion rates,and promoted gastrointestinal motility in dyspeptic mice(P<0.05).Moreover,Compound Zhizhuxiang Gel Plaster increased the diversity of gut microbiota in dyspeptic mice.A total of 30 differential metabolites were identified in the intestinal contents of young mice,and Compound Zhizhuxiang Gel Plaster could restore metabolic disturbances.Five metabolic pathways,including arginine and proline metabolism,were i-dentified as the most significantly altered after intervention.Conclusion:Compound Zhizhuxiang Gel Plaster can improve food intake and promote gastrointestinal motility in dyspeptic young mice.Its mechanism may be closely related to the regulation of gut microbiota composition and arginine and proline metabolism.