漯系列小麦品种(系)品质及抗逆基因的KASP检测与分析OA

Using 4 wheat(Triticum aestivum L.)varieties(lines)as materials,the KASP marker tech-nology combined with conventional quality testing was employed to analyze the genetic composition of their quality traits and disease-resistance and stress-tolerance genes.The quality analysis indicated that Luomai83 met the standard of medium-strong gluten wheat,while the other materials were of medium-gluten type.The KASP test revealed that in terms of quality genes,all 4 varieties contained the hard type gene Pinb-D1b and the high-quality subunit gene Ax1/Ax2*.Luomai83 combined Dx5+Dy10 with the Ax1/Ax2*high-quality subunit gene,explaining the molecular mechanism of its strong gluten phenotype.In terms of flour color,all 4 varieties carried the gene Lox-B1a with the enhancing whitening effect of high-fat oxidation enzyme activity.In terms of disease-resistance and stress-tolerance genes,the anti-spike germination gene TaSdr-B1a was distributed at a frequency of 100％in the tested materials,while the anti-leaf rust gene Lr16,the excellent allele COMT-3Ba with high lignin content,and the anti-spike germination gene Rio Blanco type were utilized to a higher extent in the 4 varieties(lines),and the anti-barley yellow rust gene Yr78,the drought-resistant gene Westonia type and TaDreb-B1a were utilized to a lower extent;no anti-leaf rust genes Lr46 and Lr48,anti-powdery mildew genes Pm21,and anti-mold blight genes Fhb1 were detected.Luomai83 contained 2 loci of high-quality subunit genes(5+10)+Ax1/Ax2*,and the quality test results were medium-strong gluten,and it also combined 4 disease-resistance and stress-tolerance genes,which could be used as excellent parental materials for wheat breeding.