西瓜品种KASP指纹图谱构建和种子纯度鉴定OA

Molecular marker technology has unique advantages in the authenticity verification and seed purity determination of plant varieties.Using the hybrid Citrullus lanatus variety Zhongnonghuang-guan No.1 and its parents as the experimental materials,competitive allele-specific PCR(KASP)markers were screened to construct the DNA fingerprint profiles of the variety and determine the seed purity.The results showed that 28 out of 32 core KASP markers were successfully typed.Based on the genotype of the loci,the Zhongnonghuangguan No.1 DNA fingerprint profiles was constructed.Among the 28 markers,6 could distinguish the father and mother plants of Zhongnonghuangguan No.1.2 polymorphic markers WmSNP69 and WmSNP89 were selected to detect the purity of the hy-brid variety,and the seed purity determination results were 95.17％and 95.14％respectively.Further,the simple repetitive sequence(SSR)markers and field identification results were consist-ent,proving that the KASP markers were feasible for the purity determination of the hybrid variety of Citrullus lanatus.The study constructed the single nucleotide polymorphism(SNP)fingerprint profiles of the Citrullus lanatus variety Zhongnonghuangguan No.1,and selected the KASP markers suitable for seed purity determination,providing technical support for the protection of variety intellectual property rights and seed quality detection.