血清miR-335-5p和miR-324-5p表达水平对肝癌患者预后的临床价值OA
Clinical value of serum miR-335-5p and miR-324-5p expression in prognosis of liver cancer patients
目的:探讨肝癌患者血清微小核糖核酸(miR)-335-5p、miR-324-5p表达水平变化,分析二者对肝癌患者预后的临床效能.方法:回顾性选取2019年8月至2021年8月收治的106例肝癌患者作为肝癌组,根据随访预后情况将肝癌组患者分为预后良好组(n=75)和预后不良组(n=31),另选取106例同期体检的健康志愿者作为对照组.采用实时荧光逆转录聚合酶链反应检测血清中miR-335-5p和miR-324-5p的表达水平,收集患者临床资料,通过Logistic回归分析影响肝癌患者预后的相关因素,并绘制受试者工作特征(ROC)曲线评估miR-335-5p、miR-324-5p对肝癌患者预后的预测效能.结果:与对照组相比,肝癌组患者血清miR-335-5p、miR-324-5p水平显著降低(P＜0.05);与预后良好组比较,预后不良组患者二者表达水平显著降低(P＜0.05).预后良好组与预后不良组患者在肿瘤直径、TNM分期、分化程度以及肝外转移方面差异有统计学意义(P＜0.05),Logistic回归分析显示,肝外转移是影响肝癌患者预后的危险因素(P＜0.05),而miR-335-5p和miR-324-5p高表达是肝癌患者预后的保护因素(P＜0.05).ROC曲线分析显示,miR-335-5p、miR-324-5p单独及联合预测患者预后的曲线下面积(AUC)分别为0.857、0.851和0.934,联合预测效能显著优于单独预测的效能(P＜0.05).结论:肝癌患者血清miR-335-5p和miR-324-5p表达水平均下调,其低表达与患者不良预后密切相关.二者联合检测对肝癌患者预后具有一定辅助预测价值.
Objective:To detect the changes in levels of serum microRNA(miRNA)-335-5p and miR-324-5p in patients with liver cancer,and further analyze the clinical efficacy of miR-335-5p and miR-324-5p in prognosis of liver cancer patients.Methods:From August 2019 to August 2021,106 liver cancer patients admitted to our hospital were included as the diseased group.They were assigned into a good prognosis group(n=75)and a poor prognosis group(n=31)according to their prognosis.Meanwhile,106 healthy volunteers who underwent physical check ups were included as the control group.qRT-PCR instrument was used to detect the relative expression levels of miR-335-5p and miR-324-5p in serum.General clinical data were collected and analyzed.Logistic regression was applied to analyze the factors influencing the prognosis of liver cancer patients.Receiver operating characteristic(ROC)curve was plotted to analyze the clinical value of miR-335-5p and miR-324-5p in predicting the prognosis of liver cancer patients.Results:Compared with the control group,the levels of serum miR-335-5p and miR-324-5p in the diseased group were obviously lower(P＜0.05).Compared with the good prognosis group,the levels of serum miR-335-5 and miR-324-5p were obviously lower in the poor prognosis group(P＜0.05).The differences in tumor diameter,TNM staging,differentiation degree,and extrahepatic metastasis between the good prognosis group and the poor prognosis group were statistically obvious(P＜0.05).Extrahepatic metastasis was a risk factor affecting the prognosis of liver cancer patients(P＜0.05),while miR-335-5p and miR-324-5p were protective factors for the prognosis of liver cancer patients(P＜0.05).The AUC of miR-335-5p and miR-324-5p alone and in combination in predicting patient prognosis was 0.857,0.851,and 0.934,respectively.The combined prediction was obviously better than miR-335-5p(Z=2.154,P=0.031)and miR-324-5p(Z=2.134,P=0.033)alone.Conclusion:The levels of serum miR-335-5p and miR-324-5p are reduced in patients with liver cancer,they are protective factors for poor prognosis and have certain auxiliary predictive value for prognosis.
黄洪华;田思源;徐虎;周元;葛方红
南通市肿瘤医院介入科(江苏 南通,226361)南通市肿瘤医院介入科(江苏 南通,226361)南通市肿瘤医院介入科(江苏 南通,226361)南通市肿瘤医院肝胆胰外科南通市肿瘤医院放疗科
医药卫生
肝癌微小核糖核酸-335-5p微小核糖核酸-324-5p预后
liver cancermicroribonucleic acid-335-5pmicroribonucleic acid-324-5pprognosis
《中西医结合肝病杂志》 2026 (2)
159-163,5
南通市自然科学基金(No.JCZ2023017)
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