小麦盐胁迫抗性相关bHLH转录因子生物信息学分析与功能预测OA

The bHLH transcription factors play important roles in the responses of crops to environ-mental stress and during their growth and development.To further study the functions of bHLH transcription factors in the salt stress response of wheat(Triticum aestivum L.),transcriptome se-quencing was conducted on the salt-tolerant material Cangmai6005 and the salt-sensitive material Kenong9204.The salt-related bHLH transcription factors were screened and subjected to bioinformat-ics analysis.The tested materials were treated with salt stress,and RNA was extracted from the root meristematic tissues.The cDNA was reverse-transcribed,and fluorescence quantitative PCR was per-formed to verify 11 key candidate genes.The results showed that a total of 33 salt-related bHLH tran-scription factors were screened out,and the encoded proteins were mainly located in the nucleus.The secondary structure was mainly composed of random coils and a-helices.The proteins were all hydro-philic.The phosphorylation sites were mainly serines.The promoter regions contained various ele-ments related to hormone response and stress induction.The analysis of the temporal and spatial ex-pression patterns of the candidate genes indicated that they were expressed in certain tissues,with the root being the main site.After salt stress treatment,the expression levels of 2 out of 11 key candidate genes significantly increased,and 2 genes significantly decreased,which was consistent with the re-sults of the transcriptome sequencing.These genes could mediate the salt tolerance of wheat.