联合转录组学与代谢组学探究嫁接对茶树脂质代谢的影响OA

In order to explore the effect of grafting on the lipid metabolism in Camellia sinensis plants,the Yuntao variety of Camellia sinensis with 1 bud and 2 leaves after grafting and the Yuntao variety of Camellia sinensis that had not undergone grafting were used as test materials.Using transcriptom-ics and metabolomics techniques in combination,the mechanism of lipid substance changes of Camel-lia sinensis after grafting was analyzed.The results showed that 2 281 differentially expressed genes and 105 differentially expressed metabolites were screened out for both the grafted Yuntao and the un-grafted Yuntao plants of Camellia sinensis.After grafting,980 genes in the plants of Camellia sinen-sis showed increased expression levels,while 1 301 genes showed decreased expression levels.The content of 61 metabolites increased,and 44 metabolites decreased.Among them,the content of 15 lipid metabolites increased,and 1 decreased.Through the KEGG pathway enrichment analysis of transcriptomics and metabolomics,732 differentially expressed genes and 24 differentially expressed metabolites were annotated to 30 pathways.These pathways mainly include metabolic pathways,bio-synthesis of secondary metabolites,and biosynthesis of phenylpropanoids,etc.In the glycerophos-pholipid metabolism pathway,lipid substance glycerophosphate choline GPC and 8 differentially ex-pressed genes such as LOC114266827 had a certain regulatory relationship;in the ether lipid metabo-lism pathway,lipid substance glycerophosphate choline GPC and 4 differentially expressed genes such as LOC114259844 had a certain regulatory relationship.