人工栽培奇楠和野生沉香化学成分差异分析OA

To explore the differences in characteristic components between cultivated Aquilaria sinensis Qi-Nan and wild Aquilaria sinensis,the ethyl ether ultrasonic extraction method was used to extract the Jinsiyou,Youyezi,Jinshaye of cultivated Aquilaria sinensis Qi-Nan,and wild Aquilaria sinensis samples for gas chromatography-mass spectrometry(GC-MS)characteristic component detection and analysis.The cell toxicity and antibacterial activities of the ethyl ether extracts from the Aquilaria sinensis samples were determined using the MTT colorimetric method and filter paper agar diffusion method.The results showed that the yield of ethyl ether extracts varied significantly among different materials.A total of 25 chemical components were detected in the 4 Aquilaria sinensis samples,mainly including sesquiterpenes and ketone compounds.Compared with wild Aquilaria sinensis,the sum of the relative contents of ketone compounds 2-(2-phenylethyl)ketone and 2-[2-4-(methoxy phe-nyl)ethyl]ketone in cultivated Aquilaria sinensis was higher,but the types of sesquiterpenes identified and the total relative content were lower.In terms of biological activity,the ethyl ether extract of Youyezi showed higher inhibitory activity against human chronic myeloid leukemia cells(K-562)and human cervical cancer cells(HeLa),and the ethyl ether extract of Jinsiyou showed stronger inhibitory ability against Staphylococcus aureus.It indicated that the chemical components of the eth-yl ether extracts from cultivated Aquilaria sinensis and wild Aquilaria sinensis were mainly sesquiter-penes and ketone compounds,but there were differences in the content and activity of characteristic components.