层积处理对北沙参种子休眠解除的激素调控机制研究OA

By dynamically monitoring the changes in endogenous hormone balance during the process of seed dormancy breaking of Glehnia littoralis,the progress of embryo morphological after-ripening,and the evolution of germination characteristics,the temporal correlation and interaction relationships among these 3 aspects were systematically revealed,thus systematically elucidating the mechanism of seed dormancy breaking,which provided theoretical basis and technical support for the standardiza-tion and large-scale cultivation of Glehnia littoralis.The quality of Glehnia littoralis seeds was in-spected,and the seeds were treated with 2 methods:sand layering and low-temperature(5℃)bare layering.The dynamic changes in germination indicators,embryo rate,and endogenous hormone con-tents were systematically compared.The results showed that the quality of this batch of Glehnia litto-ralis seeds was stable and reliable,fully meeting the quality standards required for large-scale produc-tion.Layering treatment could effectively break seed dormancy.Among them,low-temperature bare layering had a better effect.After 56 days of treatment,the germination rate could reach 100％,and it significantly promoted the increase of embryo rate,completing the morphological after-ripening.From the physiological mechanism perspective,the content of inhibitory hormone ABA continuously decreased,while the contents of promoting hormones(GA3,IAA,BR,CTK)and the ratio of(GA3+IAA+BR+CTK+ETH)/ABA increased significantly,and bare layering treatment could in-duce the hormone balance state more quickly.It indicated that 5℃ bare layering for 56 days was the best method for breaking the dormancy of Glehnia littoralis seeds,and its essence was to regulate the endogenous hormone network and coordinate the release of morphological physiological dormancy.