烟草PILS基因家族鉴定及表达模式分析OA

PIN-LIKE(PILS)proteins are a type of auxin transport proteins,of which specific localiza-tion on the cell membrane determines the direction of auxin flow between cells and the distribution of auxin content.To explore the functions of the PILS gene family of Nicotiana tabacum,using common Nicotiana tabacum as the research object,the NtPILS gene family was systematically identified at the whole-genome level,and its evolutionary relationship,physicochemical properties,chromosomal lo-calization,promoter cis-acting elements,tissue expression patterns,and response patterns under non-biological stress were analyzed using bioinformatics methods.The results showed that a total of 16 NtPILS genes were identified,among which 15 were distributed on 9 chromosomes(3,4,17,etc.),and another 1 was without a clear chromosomal localization.The transmembrane structure prediction indicated that the NtPILS protein contained 7 to 10 transmembrane structures.The promoter element analysis revealed that the NtPILS gene was rich in non-biological stress response,light response,and hormone response elements.The 14 NtPILS genes showed tissue-specific expression in different or-gans of Nicotiana tabacum(roots,stems,leaves,flowers,etc.).Most of the NtPILS genes respon-ded to low temperature,drought and salt stress.