青海省门源地区高原鼠兔携带6种人兽共患病原的分子生物学调查OA

To understand the prevalence of zoonotic pathogens in plateau pikas(Ochotona curzoniae)in Menyuan County,Qinghai Province,and to gain insights into the characteristics of pathogen carriage in high-altitude ecosystems of the Qinghai-Tibet Plateau,a molecular investigation was conducted.A total of 60 plateau pikas were captured in northern Qinghai-Tibet Plateau(Menyuan County).DNA was extracted from spleen,lung,and intestinal content samples,and polymerase chain reaction(PCR)combined with Sanger sequencing was used for molecular detection of multiple pathogens.Subsequent sequence alignment and phylogenetic analysis were performed.The results showed that the detection rates were:Brucella 3.3%(2/60),Cryptococcus 10.0%(6/60),Mucor 3.3%(2/60),Eimeria 65.0%(39/60),and Cystoisospora 10.0%(6/60),while Yersinia and Toxoplasma were not detected(0/60).Further species identification revealed that the Brucella species was Brucella rhizomaticola;the Cryptococcus species included C.neoformans and C.laurentii;the Mucor species was Rhizomucor pusillus;the Cystoisospora species was C.suis;and Eimeria showed high genetic diversity,with three independent evolutionary clades and one clade homologous to Eimeria banffensis.These findings indicate that plateau pikas can harbor multiple zoonotic pathogens and may pose a transmission risk to humans,potentially threatening public health.This study provides valuable scientific evidence for understanding the transmission mechanisms of zoonotic diseases and for the development of control strategies in Northwest China.