新疆和云南地区节肢动物的RNA病毒组学分析OA

Arthropod-borne viruses are transmitted through arthropods and pose threats to both human health and livestock production.To investigate and monitor the diversity of arboviruses under the unique geographical and climatic conditions of Xinjiang and Yunnan,this study applied high-throughput sequencing(HTS)combined with bioinformatics to perform metaviromic analysis of tick samples collected from farms in Jinghe and Wenquan Counties,Xinjiang Uygur Autonomous Region,and mosquito,fly,and sandfly samples collected from farms in Dali Bai Autonomous Prefecture,Yunnan Province.A total of 204 arthropod samples yielded 26 viral families/orders and 35 complete or nearly complete viral Contigs,including 8 vertebrate-associated viral families.Among these,5 Contigs shared less than 90%amino acid sequence identity in the RNA dependent RNA polymerase(RdRp)-encoding region with any annotated viral sequences in the non-redundant protein sequences(NR)database.Viral diversity and abundance were highest in mosquito samples from Yunnan,dominated by the families Rhabdoviridae and Mesoniviridae,while tick samples from Xinjiang were dominated by Bunyavirales and Chuviridae.Notably,Nam dinh virus detected in Yunnan mosquito samples showed high homology(>97%or>99%)with strains previously reported in encephalitis patients in Vietnam,as well as strains detected in Shenzhen and Yunnan.Phylogenetic analysis revealed that some of the newly identified viruses clustered into independent branches with unclassified viruses,suggesting potential novel viral genera.These results demonstrate that arthropods from farms in Xinjiang and Yunnan harbor viruses with rich genetic diversity,including potential new genera and risks of cross-host transmission.This study provides a scientific basis for safeguarding livestock production and public health security.