中药复方与头孢类抗生素联用治疗奶牛乳房炎MRSA研究OA
Study on the in vitro Synergistic Antibacterial Effect of the Combination of Traditional Chinese Medicine Compound and Cephalosporin Antibiotics on Multi-drug Resistant Staphylococcus aureus in Bovine Mastitis
[目的]为减少兽用抗生素使用,探究中药复方与头孢类抗生素联合使用对奶牛乳房炎多重耐药金黄色葡萄球菌(MRSA)的体外协同抑菌效果,筛选增效减毒的联合用药方案.[方法]本研究从临床发病奶牛乳房分泌物中分离鉴定MRSA菌株,采用微量肉汤稀释法测定中药复方(由蒲公英、金银花、连翘等6 味中药组成)与头孢噻呋、头孢喹肟2 种头孢类抗生素的单独抑菌浓度(MIC),通过棋盘稀释法检测联合用药的协同抑菌指数(FICI),并观察不同药物组合对MRSA生长曲线的影响.[结果]分离的MRSA菌株对头孢噻呋、头孢喹肟的单独MIC分别为64、32 μg/mL,中药复方单独MIC为256 mg/mL;中药复方与头孢噻呋、头孢喹肟联用的FICI分别为0.38、0.25,均呈显著协同抑菌作用;联合用药组对MRSA生长的抑制作用强于单独用药组,可缩短抑菌起效时间,延长抑菌持续期.[结论]中药复方与头孢类抗生素联用能显著降低后者对MRSA的抑菌浓度、增强抑菌效果,可减少临床头孢类抗生素使用剂量,为治疗奶牛乳房炎耐药菌株感染提供了新用药思路与试验依据.
[Objective]To reduce the use of veterinary antibiotics,explore the in vitro synergistic antibacterial effect of the combination of traditional Chinese medicine compound and cephalosporin antibiotics on multi-drug resistant Staphylococcus aureus(MRSA)in dairy cows with mastitis,and screen combined medication regimens that enhance efficacy and reduce toxicity.[Method]In this study,MRSA strains were isolated and identified from the breast secretions of clinically affected dairy cows.The minimum inhibitory concentrations(MIC)of a traditional Chinese medicine compound(consisting of six traditional Chinese medicines such as dandelion,honeysuckle,and forsythia)and two cephalosporin antibiotics,ceftiofur and cefquinome,were determined by the micro-broth dilution method.The synergistic antibacterial index(FICI)of the combined medication was detected by the checkerboard dilution method,and the effects of different drug combinations on the MRSA growth curve were observed.[Result]The individual MIC of the isolated MRSA strains for ceftiofur and cefquinome were 64,32μg/mL respectively,and the individual MIC of the traditional Chinese medicine compound was 256 mg/mL.The FICI of the combination of the traditional Chinese medicine compound with ceftiofur and cefquinome was 0.38 and 0.25 respectively,both showing significant synergistic antibacterial effects.The inhibitory effect of the combined medication group on the growth of MRSA was stronger than that of the single medication group,which could shorten the onset time of the antibacterial effect and prolong the duration of the antibacterial effect.[Conclusion]The combination of traditional Chinese medicine compound and cephalosporin antibiotics can significantly reduce the inhibitory concentration of the latter against MRSA,enhance the inhibitory effect,and decrease the clinical dosage of cephalosporin antibiotics.This provides a new medication idea and experimental basis for the treatment of drug-resistant strain infections in dairy cow mastitis.
杨胜男;段笑笑;刘枢清;刘欣;瞿红颖;李彦
青岛市动物疫病预防控制中心,山东 青岛 266000青岛市动物疫病预防控制中心,山东 青岛 266000青岛市动物疫病预防控制中心,山东 青岛 266000河北远征药业有限公司,河北 石家庄 050041河北远征药业有限公司,河北 石家庄 050041青岛市动物疫病预防控制中心,山东 青岛 266000
中药复方头孢类抗生素奶牛乳房炎多重耐药金黄色葡萄球菌协同抑菌作用
Chinese herbal compoundcephalosporin antibioticsdairy cow mastitismultidrug-resistant Staphylococcus aureussynergistic bacteriostatic effect
《中国乳业》 2026 (2)
29-35,7
青岛市科技惠民示范专项(25-1-5-xdny-30-nsh)
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