杜仲皮与杜仲叶对抑郁症大鼠炎症反应及海马ZO-1/Occludin的作用比较OA
Comparative effects of Eucommia ulmoides bark and leaf on inflammatory response and hippocampal ZO-1/Occludin in depressive rats
目的 探讨比较杜仲皮及叶对抑郁症模型大鼠炎症和血脑屏障损伤的作用及特点.方法 采用慢性不可预见温和应激(CUMS)建立抑郁症大鼠模型,SD大鼠随机分为对照组、抑郁组、杜仲皮组、杜仲叶组,分析进行旷场、Morris水迷宫实验检测分析行为学相关指标;苏木素-伊红(HE)、Nissl染色观察大鼠海马(HP)病理形态;ELISA法检测大鼠血清、海马中IL-1β、IL-6、TNF-α含量;免疫荧光法检测ZO-1、Occludin的表达水平.结果 与对照组比较,抑郁组大鼠目标象限停留时间、穿越平台、爬格次数、直立次数明显降低(P＜0.05或P＜0.01);海马组织存在明显的炎细胞浸润、尼氏体减少甚至消失;IL-1β、IL-6、TNF-α含量明显升高(P＜0.01);ZO-1、Occludin阳性表达降低,荧光强度减弱(P＜0.05).与抑郁组比较,杜仲皮组、杜仲叶组大鼠、目标象限停留时间、穿越平台、爬格次数、直立次数明显升高(P＜0.05或P＜0.01);病理形态明显改善;杜仲皮、杜仲叶组大鼠血清、海马中IL-1β、IL-6、TNF-α含量明显降低(P＜0.01或P＜0.05);海马组织ZO-1、Occludin阳性表达升高,荧光强度升高(P＜0.05).结论 杜仲皮、杜仲叶均能明显改善抑郁症大鼠行为,调节血脑屏障通透性,减轻炎症反应损伤,从而保护神经功能.
Objective Exploring the effects and characteristics of Eucommia ulmoides(E.ulmoides)bark and leaveson inflammation and blood-brain barrier damage in rats with depression.Methods the chronic unpredictable mild stress(CUMS)was used to establish the rat model of depression,the SD rats were randomly divided into control group,depressed group,E.ulmoides bark group and E.ulmoides leaf group.Behavioral tests were carried out by using open field and Morris water maze experiments;hematoxylin-eosin(HE)and Nissl staining were used to observe the pathological morphology of the hippocampus(HP)of the rats;ELISA was used to detect the contents of IL-1β,IL-6,and TNF-α in the serum and hippocampus of the rats;The expression levels of ZO-1 and Occludin were detected by immunofluorescence.Results Compared with the control group,the target quadrant residence time,crossing platforms,the number of climbing frames and standing frequency of rats in the depressed group were significantly lower(P＜0.05 or P＜0.01);There are obvious cellular structural blurriness,inflammatory cell infiltration and damage,decreased or even disappeared Nissl bodies,and degeneration and necrosis of nerve cells in hippocampal tissue;the contents of IL-1β,IL-6,and TNF-α were significantly elevated(P＜0.01);the positive expression of ZO-1 and Occludin was reduced,and the fluorescence intensity was reduced(P＜0.05).Compared with the depressed group,the residence time in the target quadrant,the number of crossing platforms,and the number of climbing frames of rats in the E.ulmoides bark group were significantly higher(P＜0.05 or P＜0.01),the number of crossing platforms,the number of climbing frames and standing frequency ofrats in the E.ulmoides leaf group were significantly higher(P＜0.05 or P＜0.01);the pathological morphology was restored;and the contents of IL-1β,IL-6,and TNF-α in serum and hippocampus of the rats in the E.ulmoides bark and E.ulmoides leaf group were significantly lower(P＜0.01 or P＜0.05);positive expression of ZO-1 and Occludin was elevated(P＜0.05).Conclusions Both E.ulmoides bark and E.ulmoides leaves can significantly improve the behavior of depressed rats,regulate blood-brain barrier permeability,reduce inflammatory response damage,and thus protect neurological function.
陈雨立;王鑫;刘羽;赵洪庆;易亚乔;周伟良;王汝琴;刘林
湖南中医药大学第一附属医院,长沙 410007||湖南中医药大学,长沙 410208湖南中医药大学第一附属医院,长沙 410007||湖南中医药大学,长沙 410208湖南中医药大学第一附属医院,长沙 410007湖南中医药大学,长沙 410208湖南中医药大学,长沙 410208湖南神舟医药有限公司,湖南张家界 427200张家界国仲生物科技有限公司,湖南张家界 437200湖南中医药大学第一附属医院,长沙 410007||湖南中医药大学,长沙 410208||益阳市第一中医医院,湖南益阳 413000
生物科学
抑郁症血脑屏障炎症反应杜仲
depressionblood-brain barrierinflammatory responseEucommia ulmoides
《中国实验动物学报》 2026 (2)
195-205,11
湖南省重点研发项目(2023DK2005,2024JK2121),湖南省自然科学基金(2025JJ80956,2023JJ60488),湖南省科技特派员项目(2024RC8215),产学研横向课题(2024430119000475,2024430119000477,2024430119000478,2024430119000474),湖南省教育厅项目(22A0262,24B0344).Funded by Key Research and Development Project of Hunan Province(2023DK2005,2024JK2121),Natural Science Foundation of Hunan Province(2025JJ80956,2023JJ60488),Science and Technology Commissioner Project of Hunan Province(2024RC8215),Industry-University-Research Horizontal Projects(2024430119000475,2024430119000477,2024430119000478,2024430119000474),Education Department of Hunan Province(22A0262,24B0344).
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