宣和猪ZFAT基因外显子7多态性及其对胴体和肉质性状的影响OA

To identify the polymorphisms of exon 7 in the ZFAT gene and its effect on meat traits in Xuanhe pigs,this study used first se-quencing technology to detect single nucleotide polymorphisms(SNPs)in exon 7 of the ZFAT gene in 106 Xuanhe pigs.Different genotypes and haplotype combinations were then associated with carcass and meat quality traits using a least squares model.The results showed that one missense mutation site(G3174A)and four synonymous mutation sites(C2693T,C2834T,T3173C,and C3224T)were identified in the ex-on 7 region of the ZFAT gene,and all were in a Hardy-Weinberg equilibrium state(P＞0.05).Except the C2693T and G3174A sites,the C2834T,T3173C and C3224T sites exhibited moderate polymorphism(0.3076≤PIC≤0.3739)and rich genetic diversity(0.3797≤H≤0.497 8)in Xuanhe pigs.The results of the association analysis showed that the C2693T,C2834T and T3173C sites were significantly asso-ciated with average backfat thickness and ham percentage(P＜0.05 or P＜0.01)in Xuanhe pigs.The C2693T,C2834T,T3173C and G3174A sites were significantly associated with meat color score,pH value,drip loss,and intramuscular fat content(P＜0.05 or P＜0.01)in Xuanhe pigs.Different haplotype combinations were significantly associated with carcass traits(carcass straight length,carcass diagonal length,ham percentage,lean percentage,dressing percentage),and meat traits(meat color score,marbling score,pH value,cooked rate,and drip loss)(P＜0.05 or P＜0.01)in the pigs.Meanwhile,the individuals carrying CCCGT and CCTGC haplotypes exhibited high carcass and meat quality.These results indicated that polymorphisms of exon 7 in ZFAT gene were significantly associated with carcass and meat traits in Xuanhe pigs,which would serve as a theoretical basis for using this gene marker to improve the meat quality traits in pigs.