羊口疮病毒四川流行株B2L基因及抗原表位分析OA
Analysis of the B2L Gene and Antigenic Epitopes of Sichuan Strains of Orf Virus
为了解四川省羊口疮病毒(ORFV)流行株B2L基因的分子特征,对从凉山州、眉山市及简阳市山羊养殖场采集的8份阳性样品进行ORFV B2L基因序列及抗原表位分析.结果显示:6条B2L基因序列在进化树上聚为两支;B2L蛋白序列包含8个线性B细胞表位、9个细胞毒性T细胞表位和4个辅助型T细胞表位,其中5个线性B细胞表位、7个细胞毒性T细胞表位和3个辅助型T细胞表位在国内流行毒株中的保守度大于90％.结果表明,当前四川省3个地区流行毒株的ORFV B2L基因呈多样性遗传特征,其关键抗原表位保守性较高.本研究结果丰富了四川省ORFV流行毒株的分子特征,也为ORFV感染防控和新型疫苗研发提供了参考.
In order to investigate the molecular characteristics of the B2L gene of Orf virus(ORFV)prevalent in Sichuan province,the sequences and antigenic epitopes of ORFV B2L gene were analyzed based on 8 positive samples collected from goat farms in Liangshan prefecture,Meishan city and Jianyang city.The results showed that 6 sequences of B2L gene clustered into 2 distinct branches on the phylogenetic tree;8 linear B-cell epitopes,9 cytotoxic T-cell(CTL)epitopes and 4 helper T-cell(HTL)epitopes were contained in the sequences of B2L protein.Compared with domestic ORFV reference strains,the conservation of 5 linear B-cell epitopes,7 cytotoxic T-cell epitopes and 3 helper T-cell epitopes was greater than 90％.In conclusion,the ORFV B2L gene of the strains prevalent in 3 regions in the province presented genetic diversity,and its key antigenic epitopes were highly conserved.By the study,the molecular characteristics of ORFV strains prevalent in Sichuan province were enriched,and a reference was provided for future prevention and control of ORFV infection as well as development of novel vaccines.
沈少鹏;曹琪慧;岳华;汤承
西南民族大学,四川成都 610000西南民族大学,四川成都 610000西南民族大学,四川成都 610000西南民族大学,四川成都 610000
农业科技
羊口疮病毒B2L基因抗原表位保守性四川省
ORFVB2L geneantigenic epitopeconservationSichuan province
《中国动物检疫》 2026 (2)
29-33,61,6
"十四五"国家重点研发计划项目(2021YFD 1600203)西南民族大学基本科研业务费专项(ZYN2024180)
评论