2020-2024年山东省聊城市主要牛羊养殖区口蹄疫抗体监测OA

In order to evaluate the immune level and infection status of foot-and-mouth disease(FMD)serotypes O and A in cattle and sheep in Liaocheng City,Shandong Province from 2020 to 2024,14 111 serum samples were collected from 4 counties/cities/districts where the farming of cattle and sheep was intensive,tested using ELISA,then categorized and analyzed by years,regions,species,serotypes and farming scale.Chi-square test or Cochran-Mantel-Haenszel(CMH)stratified test was used for intergroup comparison,and Spearman analysis was for correlation.The results revealed that the average qualification rate of FMD serotypes O/A antibodies in cattle(94.55％/81.82％)was higher than that in sheep(93.13％/81.16％),with a statistically significant difference(P＜0.05);all the qualification rates of FMD serotypes O/A antibodies in cattle and sheep across all years and regions exceeded 70％minimum standard that was defined by the Ministry of Agriculture and Rural Affairs,but the qualification rate of serotype O antibody was generally higher than that of serotype A(P＜0.05),with lower dispersion.FMD immunity was affected(P＜0.05)by regions,years and farming scale,and the qualification rate was lower in some regions and years,higher on intensive farms(93.18％),compared to smallholders(88.99％),with a statistically significant difference(P＜0.01).The total positivity rate of non-structural protein(NSP)antibody was 4.17％,higher in cattle(6.23％)than in sheep(2.11％);the qualification rate of FMD serotype O antibody in cattle was inversely correlated(Rho=-0.550,P=0.019)with the positivity rate of NSP antibody.In conclusion,the level of immune antibody for FMD serotypes O and A in major cattle and sheep farming areas in Liaocheng City was generally meet the required standards,the immunity was better in cattle than in sheep,superior against serotype O to serotype A,and preferable on large-scale farms compared to smallholders;and FMD could be effectively controlled through improving qualification rate of FMD antibody.It was recommended to continuously strengthen compulsory FMD vaccination and antibody level monitoring to safeguard high antibody level of the cattle and sheep populations across the region,and thereby to prevent any occurrence of FMD outbreak.