基于代谢组学探究原花青素改善酒精肝机制OA

Objective To explore the target and molecular mechanism of procyanidin B2(PB2)in the treatment of alcoholic liver damage(ALD),and to provide a theoretical basis for the clinical intervention of ALD.Methods The ALD model of mice was established by intragastric administration of 40%alcohol solution[10 ml/(kg·d)].The normal control group(NC group),ethanol model group(AL group),silybin positive control group(GL group),high dose PB2 group(PBH group)and low dose PB2 group(PBL group)were set up,with 6 mice in each group.Alanine amino-transferase(ALT),aspartate transferase(AST),triglyceride(TG),total cholesterol(TC)and alcohol metabolism related markers in plasma were detected.LC-MS/MS technology was used for plasma metabolomics analysis,and KEGG enrichment analysis was used to screen the key metabolic pathways of PB2 intervention.Results The data showed that PB2 could significantly reduce the levels of ALT,AST and TG in ALD model mice(P<0.05).At the same time,the markers related to alcohol metabolism were improved,thereby increasing the NAD+/NADH ratio.Metabolomics analysis showed that PB2 could improve the lipid metabolism disorder caused by ALD by regulating metabolic pathways such as alcoholism and steroid hormone biosynthesis.Conclusion PB2 can significantly reduce the level of liver enzymes in ALD mice,improve alcohol metabolism markers,and regulate related metabolic pathways.It provides a theoretical basis for natural compounds to intervene in ALD,and also provides new ideas for related drug research and development.