低洼盐碱地稻蟹综合种养不同时期水稻根际土壤微生物群落动态变化OA
Dynamic variations of rice rhizosphere soil microbial community at different stages of integrated rice-crab coculture in low-lying saline-alkali land
研究低洼盐碱地稻蟹综合种养不同时期水稻根际土壤微生物群落多样性、结构组成以及土壤理化性质的动态变化,可为盐碱地改良和作物优质生长提供重要理论依据.采用 Illumina Miseq技术对水稻种植前、分蘖期、抽穗期和成熟期的根际土壤细菌 16S rRNA的V3—V4 区和真菌的ITS1-1F区进行测序.分析结果表明,与水稻种植前相比,其它 3 个生育期的根际土壤微生物群落均存在显著差异,分蘖期与抽穗期、成熟期之间也存在显著差异,抽穗期之后趋于稳定,且抽穗期时菌群多样性和丰度最高.不同时期保持着核心根际微生物类群,但丰度各有差异,变形菌门、拟杆菌门、芽单胞菌门和酸杆菌门为主要的细菌优势菌门;子囊菌门、罗兹菌门和壶菌门为主要的真菌优势菌门.在种植前,细菌群落的特征微生物是放线菌门、绿弯菌门和变形菌门;抽穗期的特征微生物是纳古菌门和疣微菌门;成熟期的特征微生物是拟杆菌门、脱硫杆菌门和粘球菌门.真菌群落中,种植前的特征微生物是被孢霉门;成熟期的特征微生物是子囊菌门.土壤 pH 和电导率随着水稻的生育进程而降低,土壤总盐分水平也随之降低.低洼盐碱地稻蟹综合种养模式下,水稻根际土壤微生物群落构成以及多种土壤理化因子和土壤酶活性均随着水稻的生育进程展现出动态变化的特征.
The study examines the dynamic variations in the diversity and structural composition of the rice rhizosphere soil microbial community,as well as the variations in soil physico-chemical properties,across different stages of integrated rice-crab coculture in low-lying saline-alkali land,which provides significant theoretical support for the amendment of saline-alkali land and the enhancement of crop quality.Illumina Miseq technology is utilized to sequence the V3-V4 region of bacterial 16S rRNA and the fungal ITS1-1F region in rhizosphere soil samples collected at the pre-planting,tillering,heading,and maturity stages of rice cultivation.The analysis indicates significant differences in the rhizosphere soil microbial communities among the other three growth stages compared to the pre-planting stage,as well as between the tillering and the heading and maturity stages,which tend to stabilize after the heading stage.The microbial community diversity and abundance are found to peak at the heading stage.A core group of rhizosphere microbial community is maintained across all stages,albeit with varying abundances.Proteobacteria,Bacteroidota,Gemmatimonadota,and Acidobacteriota are identified as the main dominant bacterial phyla,while Ascomycota,Rozellomycota,and Chytridiomycota are the main dominant fungal phyla.Characteristic microorganisms of the bacterial community at the pre-planting stage are Actinobacteriota,Chloroflexi,and Proteobacteria;at the heading stage,Nanoarchaeota and Verrucomicrobiota;at maturity,Bacteroidota,Desulfobacterota,and Myxococcota.In the fungal community,Mortierellomycota is the characteristic microorganism at the pre-planting stage,and Ascomycota,at the maturity stage.Soil pH and electrical conductivity decreases with the growth process of rice,and so does the total soil salinity level.The composition of the rhizosphere soil microbial community and multiple soil physico-chemical factors and soil enzyme activities exhibit dynamic variations in conjunction with the rice growth process under the integrated rice-crab coculture model in low-lying saline-alkali land.
孙怡欣;刘祥婷;杨涓;石伟;王彬;田蕾;罗成科;邱小琮
宁夏大学生命科学学院,银川 750021宁夏大学生命科学学院,银川 750021宁夏大学生命科学学院,银川 750021宁夏银川市水产技术推广服务中心,银川 750001宁夏大学农学院,银川 750021宁夏大学农学院,银川 750021宁夏大学农学院,银川 750021宁夏大学生命科学学院,银川 750021
农业科技
根际微生物高通量测序不同时期低洼盐碱地稻蟹综合种养土壤理化性质
rhizosphere microorganismhigh-throughput sequencingdifferent stageslow-lying saline-alkali landintegrated rice-crab coculturesoil physico-chemical properties
《生态科学》 2026 (1)
19-29,11
国家重点研发计划专题(2021YFD1900603-03)2023年大学生创新创业项目(S202310749083)
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