环保试剂在眼组织病理技术中的应用OA

Objective:To investigate the application of environmentally-sustainable reagents in ophthalmic tissue pathology techniques.Methods:We prospectively collected 190 surgical specimens submitted to the pathology department of Zhongshan Ophthalmic Center between June 2023 and March 2024.Each specimen was divided into two sets,one treated with environmentally-sustainable reagents and the other with conventional reagents for fixation,dehydration,clearing,infiltration,staining,and mounting.The quality of histological sections from both groups was evaluated and compared.Results:Compared to the traditional reagent group,environmentally-sustainable reagent group showed good dehydration performance,moderately soft and hard tissue sections with intact cut surfaces and no cracks.H&E staining sections prepared with environmentally-sustainable reagents exhibited clear nuclear structures,distinct nuclear-cytoplasmic contrast,and clean backgrounds.The excellent section rates were 97.9%and 98.4%for the two,respectively.There was no significant difference in section quality between the two groups(Z=-0.645,P=0.519).Tissue antigenicity is well-preserved during immunohistochemical procedures,with strong positive signals and accurate localization without background staining.Conclusions:The use of environmentally-sustainable reagents as a replacement for conventional reagents in the dehydration and staining of ophthalmic tissue yields satisfactory results,meeting the daily requirements for routine histological sectioning and staining.The entire process is xylene-free,environmentally-sustainable,cost-effective and worth being applied widely.