CRISPR-Cas12a系统在病原体检测领域的最新研究进展OA
Recent Advances in the CRISPR-Cas12a System for Pathogen Detection
成簇规律间隔短回文重复序列-关联蛋白12a(CRISPR-Cas12a)体系是一种源自细菌的自然免疫防御机制,因其高特异度、高灵敏度、无需依赖昂贵设备、操作简便以及成本效益显著等优点,在核酸分析领域引起广泛的关注.CRIS-PR-Cas12a系统能够与扩增技术、生物传感器、拉曼光谱、微流控等相关技术联用,从而提高检测系统的灵敏度,减少交叉污染,缩短检测时间,极大拓宽了其应用场景.近年来该技术被广泛应用于病毒、细菌、支原体等病原体核酸检测中.该文主要介绍了CRISPR-Cas12a系统在上述病原体检测中的研究进展,深入探讨了其优势和局限性,旨在推动该技术在病原体的快速识别、精准诊断及现场筛查场景中的深度应用与迭代升级的实践思考.
The clustered regulatory interspaced short palindromic repeat-associated protein 12a(CRISPR-Cas12a)system is a natural immune defense mechanism derived from bacteria,which has garnered significant attention in the field of nucleic acid analysis due to its high specificity,high sensitivity,equipment-free operation,simplicity and remarkable cost-effectiveness.The CRISPR-Cas12a system can be integrated with related technologies such as amplification techniques,biosensors,Raman spec-troscopy,and microfluidics to enhance the detection sensitivity of the system,reduce cross contamination,shorten the testing time,and broaden the application scenarios.In recent years,the CRISPR-Cas12a technology has been widely applied in nucleic acid detection for pathogens such as viruses,bacteria and mycoplasmas.This article primarily reviews the research advances of the CRISPR-Cas12a system in the aforementioned pathogen detection applications,provides an in-depth analysis of its advantag-es and limitations,and proposes practical strategies to deepen its implementation and iterative refinement in rapid pathogen iden-tification,precise diagnosis,and on-site screening scenarios.
马贤参;常新昊;司文喆
北京大学第三医院 检验科,北京 100191北京大学第三医院 检验科,北京 100191北京大学第三医院 检验科,北京 100191||北京大学第三医院 心血管分子生物学与调节肽重点实验室,北京 100191||北京大学第三医院 血管稳态与重构全国重点实验室,北京 100191
医药卫生
成簇规律的间隔短回文重复序列-关联蛋白12a系统病原体核酸检测
clustered regulatory interspaced short palindromic repeat-associated protein 12a systempathogennucleic acid testing
《现代检验医学杂志》 2026 (2)
192-197,6
北京市科技新星计划(20220484090)北京市科技新星交叉合作课题(20230484442)北京市自然科学基金(7232206)血管稳态与重构全国重点实验室研究基金资助(2024-VHR-SY-13).
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