血细胞分析仪WDF和WNB散点图在血小板数量检测质量管理中的应用OA

Objective To explore and validate the value of WBC WDF Scattergram(WDF)and WBC NRBC BASO Scattergram(WNB)in monitoring blood microagglutination,large platelets and platelet aggregation.Methods A total of 1 788 EDTA-K2 blood samples were collected from the First Affiliated Hospital of Xi'an Jiaotong University between April 2023 and December 2023.When the Mindray BC-6800 Plus automatic hematology analyzers triggered the platelet agglutination alarms,exhibited ab-normal platelet histograms,or showed abnormal Ghost regions in WDF/WNB scatter plots,the MC-80 automated morphology analyzer was used to assess the relationship between different abnormal alarms and microagglutination,platelet aggregation and large platelet.Results Among 173 samples with abnormal Ghost regions in WDF scatter plots,platelet counts decreased.Com-pared to pre-recollection levels,platelet counts significantly increased after recollection(Z=-10.009,P＜0.05).Among 1252 sam-ples with abnormal Ghost zone in WNB scatter plots,the MC-80 automatic morphology analyzer revealed large platelet in 1035 cases(82.67％),and platelet aggregation in 217 cases(17.33％).There was statistically significant difference in the mean platelet volume(MPV),large platelet count ratio(P-LCR),and platelet distribution width standard deviation(PDW-SD)were significant-ly lower in platelet aggregation samples than in large platelet samples(t=9.785,8.682,8.344,all P＜0.05).Platelet count(PCT)and large platelet count(P-LCC)were higher in platelet aggregation samples than in large platelets samples,with no statistically significant differences(t=-0.777,-1.490,all P＞0.05).Among 200 samples with large platelet,the impedance-based platelet count(PLT-I)was 100(84,127)× 109/L,while the optical platelet count(PLT-O)was 104(85,132)× 109/L.There was no statistically significant difference between PLT-I and PLT-O(Z=-0.280,P＞0.05).Among 217 platelet aggregation samples with abnormal Ghost regions on WNB scatter plots,the PLT-I was 95(77,119)× 109/L and the PLT-O was 110(89,146)× 109/L.PLT-O counts were significantly higher than PLT-I counts(Z=-2.987,P＜0.05).Conclusions The process of hematology analysis and report review should integrate information from blood cell counts,WDF and WNB scatter plots,and platelet histograms to establish reasonable rules and procedures for platelet reexamination.It will be necessary to incorporate morphological analysis results for comprehensive judgment to ensure the accuracy and reliability of hematology analysis results.