艾滋病患者血清sCD14、CGRP、FOXO3水平表达与疾病分期和免疫功能的相关性研究OA

Objective To investigate the relationship between the levels of serum soluble cluster of differentiation antigen 14(sCD14),calcitonin gene related peptide(CGRP),forkhead box transcription protein 3(FOXO3)with disease stage and immune function in acquired immune deficiency syndromes(AIDS)patients.Methods 175 AIDS patients admitted to and 175 healthy individuals under going physical examinations in Shijiazhuang Fifth Hospital and Shijiazhuang Maternal and Child Health Hos-pital from January 2021 to January 2024 were enrolled as the AIDS group and the control group,respectively.The enzyme-linked immunosorbent assay(ELISA)method was applied to detect the expression levels of sCD14,CGRP and FOXO3 in the serum of both groups.Spearman and Pearson correlation analyses were applied to analyze the correlation between sCD14,CGRP,FOXO3 levels with disease stage and immune function.Multivariate Logistic regression was used to analyze the factors influencing poor prognosis in patients with AIDS.Receiver operating characteristic(ROC)curve was applied to predict the predictive value of sCD14,CGRP and FOXO3 levels for poor prognosis in AIDS patients.Results Compared with the control group,the levels of serum sCD14(5.12±1.25 μ g/ml vs 2.83±0.46 μ g/ml),and CD8+(476.88±51.38 cells/μ l vs 396.57±35.42 cells/μ l)were significantly increased,while the levels of CGRP(42.38±14.69 pg/ml vs 76.53±18.54 pg/ml),FOXO3(5.81±1.34 ng/ml vs 8.02±1.07 ng/ml),CD4+(271.18±68.54 cells/μ l vs 728.69±88.49 cells/μ l)and the ratio of CD4+/CD8+(0.57±0.12 vs 1.20±0.15)were significantly decreased(t=17.049～54.072,all P＜0.05)in the AIDS group.The serum levels of sCD14 and CD8+in AIDS group progressively increased across the acute phase,asymptomatic phase and AIDS phase,while the levels of CGRP,FOXO3,CD4+and the ratio of CD4+/CD8+progressively decreased,with statistically significant differences(F=25.322～125.502,all P＜0.05).The sCD14 was negatively correlated with CD4+and CD4+/CD8+,and positively correlated with CD8+and disease stage.CGRP and FOXO3 were positively correlated with CD4+and CD4+/CD8+,and negatively correlated with CD8+and disease stage,with statistically significant differences(r=-0.746～0.759,all P＜0.05).The levels of sCD14,CGRP,and FOXO3 were significantly associated with genital herpes,lymphoma,and HIV viral load(t/F=2.381～39.190,all P＜0.05).The levels of serum sCD14,CD8+and HIV viral load in the poor prognosis group were significantly higher than those in the fa-vorable prognosis group.Conversely,the levels of CGRP,FOXO3,CD4+and the CD4+/CD8+ratio were significantly lower in the poor prognosis group than those in the favorable prognosis group,with statistically(t=5.071～10.577,all P＜0.05).Elevated sCD14,CD8+and HIV viral load were risk factors,while CGRP,FOXO3,CD4+and CD4+/CD8+ration were protective factors for poor prognosis in AIDS patients,with statistical significances(Wald x2=6.349～18.825,all P＜0.05).The combined AUC for predicting poor prognosis in AIDS patients using sCD14,CGRP,and FOXO3 levels was higher than the AUC for predicting poor prognosis using sCD14,CGRP,or FOXO3 alone significant differences(Z=3.757,3.836,3.353,all P＜0.05).Conclusions sCD14 is upregulated in the serum of AIDS patients,while CGRP and FOXO3 are downregulated,these markers are closely re-lated to disease stage and immune function,and their combined use demonstrates high predictive value for poor prognosis in AIDS patients.