首页|期刊导航|水产科技情报|基于线粒体细胞色素b基因的小黄鱼6个野生群体遗传特征研究

基于线粒体细胞色素b基因的小黄鱼6个野生群体遗传特征研究OA

Genetic characteristics of six wild populations of small yellow croaker(Larimichthys polyactis)based on mitochondrial Cytb gene

中文摘要英文摘要

为了解小黄鱼(Larimichthys polyactis)不同地理种群的遗传特征,为其种质资源保护和可持续开发利用提供理论基础,基于Cytb基因(线粒体细胞色素b基因)标记的方法对我国东部沿海 6 个小黄鱼群体的遗传多样性和遗传结构进行了分析.结果表明,6 个群体中共有变异位点 138 个,占 19.86%,其中简约信息位点 102 个,单独位点 36 个;共检测出 96 个单倍型,其中共享单倍型有 15 个,独有单倍型 81 个.6个群体的单倍型多样性为0.925~0.972,核苷酸多样性为0.003 48~0.035 31.分子方差分析(AMOVA)表明,群体内遗传变异指数(91.89%)高于群体间变异指数(8.11%),中性检验表明,除了霞浦群体可能有过较小的群体扩张,其他群体均比较稳定.小黄鱼群体单倍型多样性较高,但核苷酸多样性较低,总体来说群体分化程度不大,比较稳定.霞浦群体相比其他 5 个群体遗传距离较大,遗传分化程度较高.

Small yellow croaker(Larimichthys polyactis)has significant economic value in China,but its popula-tions are grappling with diminishing germplasm resources.To address this issue,a study delved into the genetic structure of six small yellow croaker populations along the east coast of China,utilizing the mitochondrial cyto-chrome b(Cytb)gene.The results revealed a total of 138 variable sites across the six populations,accounting for 19.86%of all sites.Among these,102 were parsimony-informative sites and 36 were singleton sites.Additional-ly,96 haplotypes were identified,comprising 15 shared haplotypes and 81 private haplotypes.Haplotype diversity ranged from 0.925 to 0.972,and nucleotide diversity ranged from 0.003 48 to 0.035 31.AMOVA results indica-ted higher genetic variation within populations(91.89%)compared to inter-population variation(8.11%).Neu-tral tests suggested population stability,except for the Xiapu population,which may have experienced a minor population expansion.While small yellow croaker populations exhibited high haplotype diversity,nucleotide diver-sity was comparatively low.Overall,population differentiation was minimal and stable,with the Xiapu population demonstrating greater genetic distance and higher genetic differentiation compared to the other five populations.

杜书然;祝斐;徐大凤;陈淑吟;贾超峰;孟乾;孙瑞健;高波;李忠琦

江苏省海洋水产研究所,江苏 南通 264007江苏省海洋水产研究所,江苏 南通 264007江苏省海洋水产研究所,江苏 南通 264007江苏省海洋水产研究所,江苏 南通 264007江苏省海洋水产研究所,江苏 南通 264007江苏省海洋水产研究所,江苏 南通 264007江苏省海洋水产研究所,江苏 南通 264007江苏省海洋水产研究所,江苏 南通 264007江苏省海洋水产研究所,江苏 南通 264007

小黄鱼细胞色素b基因野生群体遗传多样性

Larimichthys polyactiscytochrome b genewild populationgenetic diversity

《水产科技情报》 2026 (2)

69-75,7

长江口小黄鱼种质资源挖掘与规模化繁育技术研究(MS12022029).

10.16446/j.fsti.20240500209

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