CD147高表达促进乳腺癌细胞增殖、侵袭并增强脂代谢OA

Objective:To investigate the effects of cluster of differentiation 147(CD147)expression on the proliferation,invasion,and lipid metabolism of breast cancer cells.Methods:Breast cancer tissue samples of different molecular subtypes and their corresponding cell lines were collected.Immunohistochemistry,RT-PCR,and Western blot were performed to de-tect differences in CD147 expression.CD147 knockdown and overexpression breast cancer cell models were established.Cell proliferation,invasion,apoptosis,and lipid metabolism were assessed using the CCK-8 assay,Transwell chamber assay,flow cytometry,and lipid content detection kits,respectively.Statistical analyses were conducted using the independent sam-ples t-test or chi-square test,with a two-sided P<0.05 indi-cating statistical significance.Results:CD147 expression was significantly higher in breast cancer tissues than in adjacent normal tissues(P<0.05).High CD147 expression was corre-lated with both poorer tumor differentiation(χ2=7.453,P<0.05)and lymph node metastasis(χ2=4.554,P<0.05).Survival analysis revealed that patients with high CD147 expression exhibited shorter overall survival(χ 2=4.001,P<0.05).Furthermore,CD147 overexpression promoted breast cancer cell proliferation and invasion while inhibiting apoptosis.Furthermore,CD147 may influence lipid metabolism in breast cancer cells by regulating sterol regulatory element binding protein 1(SREBP-1),a key protein involved in lipid metabolism.Conclusion:CD147 is highly expressed in breast cancer and is associated with poor prognosis.CD147 promotes proliferation and invasion while inhibiting apoptosis in breast cancer cells,and its mechanism may involve the regulation of SREBP-1-mediated lipid metabolism reprogramming.