日粮中添加不同水平黑果枸杞叶对绵羊生长性能、养分表观消化率及血清指标的影响OA

The aimed of this experiment was to investigate the effects of adding different levels of Lycium ruthenicum leaves to the diet on growth performance,nutrient apparent digestibility and serum indeces in sheep.Forty three-month-old Do×Hu hybrid F1 male lambs with a body weight of(29.58±2.06)kg were randomly allocated into four groups with ten lambs per group.The lambs in each group were fed isoenergetic and isonitrog-enous total mixed granule diets containing 0(con-trol group),5%,10%and 15%Lycium ruthenicum leaves(dry matter basis),respectively.The pre-trial period was 7 days,and the trial period was 66 days.The results revealed that:① compared with the control group,the final body weight and average daily gain in 5%Lycium ruthenicum leaves group of sheep were sig-nificantly increased(P<0.05),and the dry matter intake was extremely significantly increased(P<0.01).②Compared with the control group,the apparent digestibility of dry matter was extremely significantly increased in 5%Lycium ruthenicum leaves group(P<0.01),the apparent digestibility of ether extract and acid detergent fibre were significantly increased(P<0.05).③ Compared with the control group,the serum aspartate aminotransferase activity was significantly increased in Lycium ruthenicum leaves each groups was extremely significantly increased(P<0.01),the serum alanine aminotransferase activity was significantly increased in 10%and 15%groups(P<0.05),and the serum creatine kinase activity was extremely signifi-cantly increased in 15%group(P<0.01).the serum glucose and high-density lipoprotein content in Lycium ruthenicum leaves each groups were extremely significantly decreased(P<0.01),and the serum triacylglyc-erol content in 5%groups was significantly decreased(P<0.05),was extremely significantly decreased in 10%and 15%groups(P<0.01).④ Compared with the control group,the serum superoxide dismutase and glutathione peroxidase activity,total antioxidant capacity,immunoglobulin A and immunoglobulin M con-tent in Lycium ruthenicum leaves each groups were extremely significantiy increased(P<0.01),and the serum malondialdehyde and immunoglobulin G content were extremely significantly decreased(P<0.01).In conclusion,under the conditions of this experiment,dietary adding with 5%-10%Lycium ruthenicum leaves level can improve the average daily gain of sheep,dry matter intake,enhance feed utilization,promote fat and energy metabolism,and strengthene immune function and antioxidant capacity in sheep,with the 5%adding level showing the most beneficial effects.