苦豆子提取物对产气荚膜梭菌的体外抑制研究OA

Sophora alopecuroides L.,which mainly consists of alkaloids with antibacterial effect,is mostly grown in the northwest region of China.Although rich in resources,it is difficult to control and treat the deadly diseases caused by Clostridium perfringens infection in livestock.In order to explore the antibacterial effect of Sophora alo-pecuroides L.on Clostridium perfringens,selected water extracts and macerations of Sophora alopecuroides L.as experimental materials for the in vitro inhibition study of Clostridium perfringens.Water decoction was used to pre-pare Sophora alopecuroides L.water extract at concentrations of 1.0,2.0,4.0 g/mL.Acid extraction method was used to extract Sophora alopecuroides L.paste and prepare extract at a concentration of 0.1 g/mL.Then,tube twofold dilution method and paper disc diffusion method were used respectively to measure the minimum inhibitory concentration(MIC)of Sophora alopecuroides L.extract on Clostridium perfringens and the diam-eter of inhibition zone of bitter bean extract on Clostridium perfringens to determine its inhibitory effect.The results showed that the drug-sensitive film of Sophora alopecuroides L.water extract had certain inhibitory effect when the concentration was 2.0 g/mL and 4.0 g/mL,with inhibition zone diameters of 8.5 mm and 10.0 mm,the Sophora alopecuroides L.paste drug-sensitive film had better inhibitory effect when the con-centration was 0.1 g/mL,and the inhibition zone diameter was 8.5 mm.Its minimum inhibitory concentration(mic)was 5.05 mg/mL;the inhibitory effect of Sophora alopecuroides L.water extract and paste on Clos-tridium perfringens.