口腔扁平苔藓患者血清ANXA1、CXCR3表达及其与病损程度、Th17/Treg平衡的关系OA

Objective:To investigate the expression of serum annexin A1(ANXA1)and C-X-C chemokine receptor 3(CXCR3)in patients with oral lichen planus(OLP),and their relationship with the lesion severity and the T helper cell 17(Th17)/regulatory T cell(Treg)balance.Methods:From July 2022 to July 2023,120 patients diagnosed with OLP in our hospital were included as the study group.Patients were clinically classified into a non-erosive group(45 cases)and an ero-sive group(75 cases).The erosive group was further subdivided based on erosion severity into a mild-to-moderate erosion group(44 cases)and a severe erosion group(31 cases).Meantime,120 volunteers who experienced physical examinations at our hospital were designated as the control group.ELISA method was used to detect the levels of ANXA1 and CXCR3 in serum.Flow cytometry was used to detect the proportions of Th17 and Treg in peripheral blood.Spearman or Pearson methods were used to analyze the correlation between serum ANXA1,CXCR3 levels with disease severity,Th17,Treg ratios,and Th17/Treg.Multivariate logistic regression was used to analyze the factors affecting the severity of erosion in patients with OLP.Receiver operating characteristic(ROC)curves were used to analyze the diagnostic value of serum ANXA1 and CXCR3 levels for the severity of erosion in patients with OLP.Results:Compared with the control group,the study group had promi-nently lower serum ANXA1 and prominently higher CXCR3(P<0.05).Compared with the non erosion group,the erosion group had prominently lower serum ANXA1 level and Treg ratio,and prominently higher CXCR3 level,Th17 ratio,and Th17/Treg value(P<0.05),and the severe erosion group had a higher degree of change in various indicators than the mild to mod-erate erosion group(P<0.05).Serum ANXA1 was prominently negatively correlated with disease severity,Th17 proportion,and Th17/Treg,and prominently positively correlated with Treg proportion(P<0.05),serum CXCR3 was prominently posi-tively correlated with disease severity,Th17 proportion,and Th17/Treg,and prominently negatively correlated with Treg pro-portion(P<0.05).The decrease in ANXA1 and Treg levels,and the increase in CXCR3,Th17,and Th17/Treg levels were risk factors for severe erosion in patients with OLP(P<0.05).The area under the curve(AUC)values of serum ANXA1 and CXCR3 alone in the diagnosis of severe erosion in patients with OLP were 0.910 and 0.892,respectively.The AUC of com-bined diagnosis was 0.967,which was prominently higher than the individual diagnosis of the two(Zcombination-ANXA1=2.161,Zcombination-CXCR3=2.466,P<0.05).Conclusion:Serum ANXA1 is decreased,and CXCR3 is increased in patients with OLP,which is closely related to the severity of lesion and Th17/Treg balance.The combined detection of ANXA1 and CXCR3 has high diagnostic value for the severity of erosion in patients with OLP.