中老年牙周炎患者龈沟液中Epstein-Barr病毒DNA载量的检测及其暴露因素研究OA

Objective:To detect the load of Epstein-Barr virus(EBV)DNA in the gingival crevicular fluid(GCF)of middle-aged and elderly patients with periodontitis and to analyze the impact of lifestyle exposure factors,including active smoking,passive smoking,and alcohol consumption,on its distribution.Methods:A total of 101 middle-aged and elderly pa-tients with periodontitis were enrolled.Epidemiological data regarding smoking,passive smoking,and drinking habits were col-lected via questionnaires.Absolute quantification of EBV DNA in GCF samples was performed using qPCR.Non-parametric tests were used to compare EBV loads across different exposure groups,and a multiple linear regression model was construc-ted to evaluate the independent effects of these factors on viral load.Results:The median EBV DNA load in GCF of the 101 patients was 677.16 IU/mL.Univariate analysis revealed that EBV loads in current smokers were significantly higher than those in non-smokers(P<0.05,r=0.25).Similarly,the passive smoking exposure group exhibited significantly higher viral loads than the non-exposed group(P<0.05,r=0.21).No significant association was found between drinking habits and EBV load(P>0.05).Multiple linear regression showed that after adjusting for age and sex,tobacco exposure remained positively associated with increased viral loads.Conclusion:Both active and passive tobacco exposure are significantly associated with elevated local EBV DNA loads in middle-aged and elderly patients with periodontitis.Tobacco exposure may serve as a criti-cal environmental risk factor for oral EBV reactivation,and lifestyle factors should be carefully considered when assessing per-iodontal viral status.