miR-1941-5p、miR-23a-5p以及miR-3102-5p在MC3T3-E1向成骨细胞分化中的表达研究OA

Objective:To systematically analyze the dynamic expression patterns of microRNAs(miR-1941-5p,miR-23a-5p and miR-3102-5p)during the entire osteogenic differentiation process of mouse pre-osteoblast MC3T3-E1 cells.Meth-ods:MC3T3-E1 cells were induced toward osteogenic differentiation using an osteogenic induction medium.On days 7 and 14 of induction,Alizarin Red staining and alkaline phosphatase(ALP)staining were performed to observe mineralized nodules and ALP activity,followed by quantitative analysis.Meanwhile,at days 0,3,7,11,and 14 of induction,the mRNA and protein expression levels of key osteogenic markers,Runt-related transcription factor 2(Runx2),ALP and osteocalcin(OCN)were de-tected by qRT-PCR and Western blotting,respectively.Within this validated differentiation model,the temporal expression profiles of the three microRNAs were assessed via qRT-PCR at the same time points.Results:After 7 and 14 days of osteo-genic induction,both Alizarin Red and ALP staining in the experimental group were significantly enhanced compared with the control group.Concurrently,the mRNA and protein expression levels of Runx2,ALP,and OCN were markedly upregulated.In contrast,the expression levels of miR-1941-5p,miR-23a-5p,and miR-3102-5p exhibited a declining trend throughout the dif-ferentiation process.Conclusion:The results indicate that miR-1941-5p,miR-23a-5p,and miR-3102-5p are downregulated during osteogenic differentiation of MC3T3-E1 cells,suggesting their potential involvement in the regulatory network of osteo-genesis.