芦丁通过抑制ROS/TXNIP/NLRP3通路减轻牙周炎大鼠牙周组织损伤和炎症反应OA

Objective:To explore the effects of rutin(RU)on periodontal tissue damage and inflammatory response in periodontitis(PD)rats by regulating the reactive oxygen species(ROS)/thioredoxin interacting protein(TXNIP)/NOD-like receptor thermal protein domain associated protein 3(NLRP3)pathway.Methods:Successful PD rats were randomly separa-ted into PD group,RU-L group,RU-H group(gavage of 40 mg/kg and 80 mg/kg RU),and RU-H+TMAO group(gavage of 80 mg/kg RU+110 mg/kg TMAO),with 12 rats in each group.Another 12 rats were stochastically selected as the NC group.The NC group and PD group were given equal doses of physiological saline by gavage,once a day for 28 days.HE staining was performed to observe pathological changes in periodontal tissue.ELISA method was used to measure the inflammatory fac-tors in periodontal tissue.Colorimetric method was performed to measure oxidative stress in periodontal tissue.DCFH-DA method was performed to detect ROS in periodontal tissue.TUNEL method was performed to measure apoptosis in periodontal tissue cells.Moreover,Western Blot was used to detect the periodontal tissue related proteins.Results:Compared with the NC group,PD group had a large amount of inflammatory cell infiltration in periodontal tissue,accompanied by alveolar bone re-sorption,collagen fiber decomposition,and connective tissue damage.The GI,AL values,periodontal tissue TNF-α,IL-1β,IL-8,IL-18,ROS,MDA levels,cell apoptosis rate,TXNIP,NLRP3,ASC,Caspase 1,IL-1β,IL-18 protein expression were clearly increased(P<0.05),and the periodontal tissue SOD and CAT were clearly decreased(P<0.05).Compared with the PD group,the RU-L and RU-H groups showed reduced infiltration of inflammatory cells,alveolar bone resorption,collagen fi-ber breakdown,and connective tissue damage in periodontal tissue.The GI,AL values,periodontal tissue TNF-α,IL-1β,IL-8,IL-18,ROS,MDA levels,cell apoptosis rate,TXNIP,NLRP3,ASC,Caspase 1,IL-1β,IL-18 protein expression were clearly decreased(P<0.05),and the periodontal tissue SOD and CAT were clearly increased(P<0.05).Compared with the RU-H group,the RU-H+TMAO group showed increased damage to periodontal tissue,the GI,AL values,periodontal tissue TNF-α,IL-1β,IL-8,IL-18,ROS,MDA levels,cell apoptosis rate,TXNIP,NLRP3,ASC,Caspase 1,IL-1β,IL-18 protein expression were clearly increased(P<0.05),and the periodontal tissue SOD and CAT were clearly decreased(P<0.05).Conclusion:RU may alleviate periodontal tissue damage and inflammatory response in PD rats by inhibiting ROS/TXNIP/NLRP3 pathway.