玉木耳多糖热水提取工艺优化及抗氧化活性研究OA

This study employed hot water extraction to isolate polysaccharides from Auricularia cornea'Yumuer'(ACP),examining the effects of extraction temperature,number of extraction cycles,liquid-to-solid ratio,and extraction duration on ACP yield.Both single-factor experiments and response surface methodology were utilized to optimize the extraction parameters and identify the optimal conditions for ACP recovery.The antioxidant activity of ACP was assessed through free radical scavenging assays.Results indicated that the optimal extraction conditions were an extraction temperature of 100℃,extraction time of 251 minutes,three extraction cycles,and a liquid-to-solid ratio of 71∶1(mL∶g).Under these conditions,the ACP yield reached 10.07％,with a deviation of 0.7％from the predicted value.The half-maximal inhibitory concentration(IC50)values for scavenging DPPH,ABTS,hydroxyl,and superoxide anion radicals were 1.87,1.13,2.19,and 0.94 mg/mL,respectively,demonstrating dose-dependent antioxidant activity.These findings suggest that ACP possesses significant antioxidant properties,providing a scientific foundation for its further development,utilization,and potential economic value enhancement.