白鲜碱对6种植物病原真菌的抗菌活性评价OA

[Objective]Botanical fungicides have become one of the ideal choices for the research and development of green fungicides due to their unique environmental-friendly characteristics,biodegradability,and efficient selectivity for specific targets.Plant secondary metabolites,such as coumarins,anthocyanins,phenols,alkaloids,terpenoids and flavonoids,etc.,exhibit excellent antifungal activities and become the main sources for the development of botanical fungicides.[Method]In this study,the stems and leaves of Clausena lansium were used as experimental materials.Separation and purification were carried out by various column chromatography and semi-preparative liquid chromatography techniques.The structure of the compound was identified by spectroscopic techniques such as 1 H NMR and 13 C NMR,and a furanocoline alkaloid component,dictamnine,was obtained.The antifungal activity of dictamnine against six kinds of phytopathogenic fungi was tested by the mycelial growth rate method.[Result]The results showed that dictamnine inhibited the mycelial growth and morphology of six plant pathogenic fungi.The EC50 values of Fusarium solani,F.oxysporum,F.proliferatum,F.graminearum,Botryosphaeria dothidea and Botrytis cinerea were 92.61,39.37,33.09,9.9,15.48 and 10.81 μg/mL,respectively.Among them,dictamnine had a significant inhibitory effect on F.gramineum,B.dothidea and B.cinerea.Observation of the mycelial morphology of the pathogen treated with dictamnine revealed that the mycelia of the pathogen showed characteristic changes such as twisting,swelling and cavities after treatment with dictamnine.[Conclusion]It indicates that dictamnine has certain effects on the growth and morphology of six plant pathogenic fungi and possesses broad-spectrum resistance.