添加硒对蛹虫草富硒的影响OA

In order to investigate the effects of different selenium sources on selenium enrichment within fruiting bodies of Cordyceps militaris,four chemical compounds of exogenous selenium(sodium selenite,nano-selenium,chelated selenium and yeast selenium)with various concentrations were added to culture media at the different growth stages(mycelial stage,color transformation stage,primordial stage and growth stage)of C.militaris.Contents of total selenium and organic selenium of the harvested fruiting bodies were measured by using o-phenylenediamine ultraviolet spectrophotometry for analysis and comparison of the effects induced by supplying exogenous selenium with a concentration gradient treatment on the selenium enrichment at four growth stages of C.militaris.The results showed clearly that all of groups treated with the supply of exogenous selenium significantly increased the content of total selenium and organic selenium in C.militaris fruiting bodies when compared with their controls.When 16 mg/L sodium selenite was added at the color transformation stage,the content of both total selenium and organic selenium of C.militaris fruiting bodies reached the maximal value,showing 115.17 μg/g with an enrichment rate of selenium of 719.79％and 105.20 μg/g with an organic conversion rate of 91.34％respectively,in which the rate of organic conversion was 2.34 times higher than that of the control.The above results should provide an important foundation for the future research and development of new and natural supplements of selenium.