松杉灵芝倍半萜合酶相关基因家族分析OA

Ganoderma tsugae is a rare and valuable species of Ganoderma in the northeast China area,because it contains abundant compounds of terpenoids.Terpene synthases are key enzymes that promote the biosynthesis of sesquiterpene and other terpene compounds.Based on our high-quality sequence previously obtained from the whole genome of G.tsugae,this study aims to explore the composition and structure of sesquiterpene synthase gene family in this important species.Through sequence and structural comparison of homologous gene among various species of fungi,it was found that there were 10 sesquiterpene synthase coding genes within the whole genome of G.tsugae,which were mainly distributed in five different sequence contigs consisting of seven gene clusters.These genes were known to encode the germacrene A synthase(Cop1 and Cop2),the α-muurolene synthase(Cop3),the δ-Cadinene synthase(Cop4)and the trichodiene synthase(TRI5),respectively,where Cop1,Cop2,Cop3 and Cop4 all carry the typical Terpene_syn_C-2 domain.TRI5 contains the Isoprenoid_Biosyn_C1 domain.The total sequence of 10 genes ranged in length from 1 178 bp to 1 478 bp,showing a number of two to five introns.Detection of SNP information revealed that nine of the above genes had a multiple number of non-synonymous substitution sites in their coding region between the different wild strains,providing thereafter new natural genetic resources for the subsequent functional study of these key genes relating to the sesquiterpene biosynthesis in G.tsugae.