砂藓RcPM19L1基因的耐旱和耐高温功能OA

The ABA-induced wheat plasma membrane polypeptide-19 protein(AWPM-19)is closely related to plant tolerance to abiotic stresses such as drought and high-temperature.Racomitrium canescens can quickly recover to its normal growth state upon rehydration after maintaining a relative water content under 10%for many years,and it also exhibits strong high-temperature tolerance.For analyzing the drought and high-temperature tolerance function of the AWPM-19 family gene RcPM19L1,which is in response to dehydration stress in R.canescens,exploring whether RcPM19L1 can enhance the drought and high-temperature tolerance characteristics of plants,providing a theoretical basis for further studying the role of AWPM-19 family genes in regulating plant stress resistance.The expression of RcPM19L1 under different treatments of dehydration and high temperature stress in R.canescens was detected using real-time quantitative PCR.The coding sequence of RcPM19L1 was cloned,transgenic RcPM19L1 overexpression Arabidopsis thaliana lines were acquired,the phenotype under drought and high-temperature stress was observed,and physiological and biochemical indexes were analyzed.RcPM19L1 is capable of responding to dehydration and high-temperature stress in R.canescens.The full-length coding sequence of RcPM19L1 is 525 bp,encoding a protein containing 174 amino acid residues,and is predicted to possess the characteristic structures of AWPM-19.The RcPM19L1 transgenic overexpression A.thaliana lines was successfully constructed.It was found there was no significant difference in the growth status between wild-type A.thaliana and transgenic plants under drought treatment.However,under high-temperature treatment,the phenotype of RcPM19L1 transgenic A.thaliana was superior to that of wild-type plants.Under high temperature stress,compared to wild-type A thaliana,in RcPM19L1 transgenic plants,the mass fraction of some osmotic adjustment substances increased,the peroxidase activity can reach up to approximately 1.75 times of the wild-type plants,and the catalase activity can reach up to approximately 1.22 times of the wild-type plants.Heterologous overexpression of RcPM19L1 in A.thaliana improves the high-temperature tolerance,but no significantly changes on the drought tolerance.