橙皮素对脂多糖诱导人肠上皮细胞氧化损伤及凋亡的影响OA

Aimed to investigate the effects of Hesperetin(HPT)on oxidative damage and apoptosis in lipopolysaccharide(LPS)-induced human intestinal epithelial cells(NCM460).Cell viability of NCM460 cells was assessed using the MTT assay to determine the optimal concentration of HPT,and finally 50,200,400 μmol/L HPT were selected for subsequent experiments.The experimental groups included the control group,LPS group and different concentrations of HPT+LPS group.Biochemical assays were conducted to measure malondialdehyde(MDA)and reactive oxygen species(ROS)levels,glutathione peroxidase(GSH-Px)activity,superoxide dismutase(SOD)activity,total antioxidant capacity(T-AOC),as well as Caspase3/9 activity in the cells.The relative expression levels of antioxidant and apoptosis-related genes mRNA were determined using fluorescence quantitative PCR.The results showed that pretreatment with different concentrations of HPT significantly reduced MDA and ROS levels,increased GSH-Px,SOD activity,T-AOC and decreased Caspase3/9 activity compared to the LPS group.The relative expression levels of antioxidant-related genes Nrf2,HO-1,NQO1,SOD1 and anti-apoptotic factor Bcl-2 mRNA were significantly upregulated,while pro-apoptotic factors Caspase3/9 and Bax mRNA were significantly downregulated.These findings indicate that appropriate concentrations of HPT can effectively inhibit oxidative damage and apoptosis in LPS-induced NCM460 cells.