山姜素调节Hippo/YAP信号通路对肾病综合征大鼠炎性损伤的影响OA
Effect of alpinetin on inflammatory injury in nephrotic syndrome rats by regulating the Hippo/YAP signaling pathway
目的:探讨山姜素调节河马(Hippo)/Yes相关蛋白(YAP)信号通路对肾病综合征(NS)大鼠炎性损伤的影响.方法:通过注射盐酸阿霉素构建NS模型,并将大鼠随机分为NS组、不同剂量山姜素组、泼尼松组、山姜素+Hippo/YAP抑制剂-维替泊芬(VP)组,并以注射生理盐水的大鼠为对照组,试剂盒检测大鼠尿蛋白含量;自动生化分析仪检测血肌酐(Scr)和尿素氮(BUN);ELISA试剂盒检测单核细胞趋化因子-1(MCP-1)、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)水平;收集双肾,测定肾脏指数,H-E染色观察肾组织病理学改变;TUNEL法检测肾组织细胞凋亡,RT-qPCR、免疫印迹检测YAP、转录共激活因子PDZ结合基序(TAZ)mRNA及蛋白表达.结果:与对照组相比,NS组尿蛋白、血Scr和BUN、IL-6、TNF-α、MCP-1、肾脏指数、病理评分、凋亡率、p-YAP/YAP、TAZ及mRNA表达明显增加;与NS组相比,不同剂量山姜素组、泼尼松组Scr、尿蛋白、BUN、IL-6、TNF-α、MCP-1、肾脏指数、病理评分、凋亡率、p-YAP/YAP、TAZ及mRNA表达明显降低,不同剂量山姜素有差异;与160 mg/kg山姜素组相比,山姜素+VP组Scr、尿蛋白、BUN、IL-6、TNF-α、MCP-1、肾脏指数、病理评分、凋亡率、p-YAP/YAP、TAZ及mRNA表达明显降低.结论:山姜素调节Hippo/YAP信号通路减轻NS大鼠炎性损伤.
Objective:To investigate the effect of alpinetin on inflammatory injury in nephrotic syndrome(NS)rats by regulating the Hippo/Yes-associated protein(YAP)signaling pathway.Methods:A NS model was established by injecting doxorubicin hydrochloride,and rats were randomly divided into NS group,different-dose alpinetin groups,prednisone group,alpinetin+Hippo/YAP inhibitor verteporfin(VP)group,and the control group which was injected with normal saline.The assay kit was used to detect the urine protein of rats;the biochemical analyzer was used to measure the serum creatinine(Scr)and blood urea nitrogen(BUN);the ELISA kit was used to detect the levels of monocyte chemoattractant protein-1(MCP-1),tumor necrosis factor-α(TNF-α),and interleukin-6(IL-6);both kidneys were collected,and the kidney index was measured;H-E staining was used to detect renal histopathological changes;the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling(TUNEL)method was used to detect apoptosis of renal tissue cells.Finally,real-time quantitative polymerase chain reaction(RT-qPCR)and Western blotting were employed to detect the mRNA and protein expressions of YAP and transcriptional co-activator PDZ-binding motif(TAZ).Results:Compared with the control group,the levels of Scr,urinary protein,BUN,IL-6,TNF-α,MCP-1,kidney index,pathological score,apoptosis rate,p-YAP/YAP,TAZ and mRNA expression in the NS group were significantly increased.Compared with the NS group,the levels of Scr,urinary protein,BUN,IL-6,TNF-α,MCP-1,kidney index,pathological score,apoptosis rate,p-YAP/YAP,TAZ mRNA and protein expression in the different-dose alpinetin groups and prednisone group were significantly reduced,and there were differences among different doses of alpinetin.Compared with the 160 mg/kg alpinetin group,the levels of Scr,urinary protein,BUN,IL-6,TNF-α,MCP-1,kidney index,pathological score,apoptosis rate,p-YAP/YAP,TAZ mRNA and protein expression in the alpinetin+VP group obviously reduced.Conclusion:Alpinetin has an effect on inflammatory injury in NS rats by regulating the Hippo/YAP signaling pathway.
孙祥龙;苏丽杰;张坤英
山东第二医科大学临床医学院||阳光融和医院老年病科国药医疗潍坊医院肾内科潍坊市人民医院肾内科,潍坊 261000
医药卫生
肾病综合征山姜素肾Hippo/YAP信号通路炎性损伤
nephrotic syndromealpinetinkidneyHippo/YAP signaling pathwayinflammatory injury
《解剖学杂志》 2026 (1)
30-35,6
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