大蒜水通道蛋白基因AsPIP1;1和AsTIP2;1的克隆、鉴定及其对盐胁迫的响应OA

Aquaporins(AQPs)are primarily responsible for the transmembrane transport of water molecules and are involved in processes such as plant growth,development,and responses to environmental stress.Garlic is an important vegetable crop widely cultivated worldwide,but there are currently few reports on salt-tolerance genes in garlic.It aimed to clone the genes AsPIP1;1 and AsTIP2;1,encoding plasma membrane intrinsic protein(PIP)and tonoplast intrinsic protein(TIP)respectively,from garlic,analyze their sequence characteristics,investigate their expression patterns under salt stress conditions,and evaluate their potential roles in garlic salt tolerance.We cloned the AsPIP1;1 and AsTIP2;1 genes from garlic using RT-PCR.Bioinformatics methods were employed to analyze the open reading frames,encode amino acid sequences,and conserve domains of the cloned genes.Furthermore,the expression profiles of AsPIP1;1 and AsTIP2;1 in different tissues and under salt stress conditions were detected u-sing Quantitative Real-time PCR.The results showed that the open reading frames of the AsPIP1;1 and AsTIP2;1 genes were 867,747 bp in length,encoding 288,248 amino acids,respectively,with the conserved NPA motifs.AsPIP1;1 and AsTIP2;1 were highly expressed in garlic leaves and roots,and salt stress strongly induced changes in the transcriptional levels of both genes.Furthermore,AsPIP1;1 and AsTIP2;1 may interact with other AQP pro-teins,cell wall-associated proteins,and transporter proteins to adapt to the salt stress environment.These results indicate that the AsPIP1;1 and AsTIP2;1 genes may be involved in the garlic plant's response to salt stress.