控制玉米叶片数的QTL定位及基因功能注释OA

To clarify the genetic characteristics of total leaf number and leaf number above the ear in maize,and reveal their regulatory effects on maize yield potential,biomass accumulation,and lodging resistance,experi-ments on QTL mapping and gene mining for controlling maize leaf number were conducted.By using SLAF-seq tech-nology for high-throughput sequencing of Chang 7-2,PHB1M,and 138 F2∶3 families,combined with two planting density treatments(E1:60 000 plants/ha;E2:120 000 plants/ha)QTL mapping was performed on leaf number phe-notypic data,and gene mining was performed on the mapping results.The results showed that two total leaf number QTLs distributed on chromosome 8 were the main effect QTLs,contribution rates were 16.96％and 23.08％,re-spectively,and the additive effect value was negative;the QTL for the leaf number above the ear distributed on chro-mosomes 2 and 4,with a positive additive effect value.the two QTLs distributed on chromosome 4 were the main effect QTL,contribution rates were 10.18％and 14.75％,respectively;the QTLs for total leaf number and leaf number above the ear were distributed in different chromosomal regions and might be subject to relatively independ-ent genetic regulation.Gene functional analysis revealed genes screened involvement in carbohydrate metabolism,plant hormone signal transduction,and selected some transcription factors.The results of this study provide richer theoretical support for further revealing the genetic basis of maize leaf number and offer targets for marker-assisted breeding.