利用BSA-seq技术挖掘油菜耐铝毒基因OA

In recent years,soil acidification and aluminum ion toxicity in southern cultivated land have intensi-fied.To explore the gene loci related to aluminum tolerance in rapeseed,the F2 population obtained by crossing the aluminum tolerant variety R248 with the aluminum sensitive variety S120 was used as the experimental material.The extreme mixed pool of aluminum toxicity tolerance was constructed by phenotypic identification,and QTL locali-zation analysis was performed by BSA-seq.The phenotype identification results showed that the aluminum toxicity tolerance of the F2 population exhibited a continuous normal distribution.After sequencing,mutation detection,and association analysis,a total of 4 intervals related to aluminum toxicity tolerance were obtained,distributed on chro-mosomes A07,C02,C03,and C08,with a total length of 0.73 Mb and 98 genes.Based on the enrichment analysis results and gene annotation information,it is predicted that BnaA07g35600D,BnaA07g35660D,BnaA07g35700D,BnaA07g3570D,and BnaC03g39670D will participate in the synthesis and transportation of aluminum tolerance hormones in rapeseed,BnaC03g39840D participates in the synthesis of cell wall pectinesterase,BnaC03g39750D and BnaC08g20580D were transcription factors with MYB structure,BnaC03g39850D and BnaC03g39980D were involved in amino acid metabolism.These genes are highly correlated with aluminum tolerance in rapeseed,laying the foundation for the cloning and functional research of aluminum tolerance genes in the next step.