不同证型早发性卵巢功能不全患者肠道菌群特征分析OA

Objective To explore the characteristics of intestinal flora in patients with premature ovarian insufficiency(POI)presenting liver-kidney yin deficiency syndrome,liver stagnation kidney deficiency syndrome,and spleen-kidney yang de-ficiency syndrome,to provide a basis for syndrome differentiation and classification in POI.Methods A total of 60 patients with POI admitted to Jiangxi Maternal and Child Health Hospital from April 2023 to August 2024 were selected as the study subjects.According to the different syndrome patterns,they were divided into the liver-kidney yin deficiency group(n=20),the liver stagnation kidney deficiency group(n=20),and the spleen-kidney yang deficiency group(n=20).Simultaneously,20 healthy volunteers were selected as the healthy control group.Serum levels of estradiol(E2),luteinizing hormone(LH),follicle-stimulating hormone(FSH),and anti-Müllerian hormone(AMH)were compared among all groups,and bioinformat-ics methods were employed to analyze the characteristics of their intestinal flora.Results There were no significant differ-ences in serum E2 and LH levels among the four groups(P＞0.05).There were statistically significant differences in the comparison of FSH and AMH levels among the four groups(P＜0.05).The FSH levels in the liver-kidney yin defi-ciency group,liver stagnation kidney deficiency group,and spleen-kidney yang deficiency group were higher than those of the healthy control group,while their AMH levels were lower than those of the healthy control group,and the differences were statistically significant(P＜0.05).The abun-dance of Firmicutes in the liver-kidney yin deficiency group was lower than that in the spleen-kidney yang deficiency group and the healthy control group,while the abundance of Proteobacteria was higher than that in the liver stagnation kidney de-ficiency group,the spleen-kidney yang deficiency group,and the healthy control group.The abundance of Actinobacteria,Blautia,Bifidobacterium,and Eubacterium hallii in the liver-kidney yin deficiency group,the liver stagnation kidney defi-ciency group,and the healthy control group was lower than that in the spleen-kidney yang deficiency group,while the abundance of Bacteroidetes was higher than that in the spleen-kidney yang deficiency group.The abundance of Lactobacil-lus in the liver-kidney yin deficiency group and the liver stagnation kidney deficiency group was higher than that in the spleen-kidney yang deficiency group and the healthy control group.The abundance of Bacteroides in the liver-kidney yin deficiency group and the healthy control group was higher than that in the liver stagnation kidney deficiency group and the spleen-kidney yang deficiency group.The abundance of Faecalibacterium and Agaribacter in the liver-kidney yin defi-ciency group,the liver stagnation kidney deficiency group,and the spleen-kidney yang deficiency group were lower than those in the healthy control group,while the abundance of Romboutsia was higher than that in the healthy control group.The abundance of Patescibacteria and Clostridium in the liver stagnation kidney deficiency group was higher than that in the liv-er-kidney yin deficiency group,the spleen-kidney yang deficiency group,and the healthy control group,with the differences statistically significant(P＜0.05).Conclusion Romboutsia may be the specific species to distinguish POI patients from healthy people.The increased abundance of Proteobacteria,the decreased abundance of Firmicutes and Blautia may be the characteristic of POI patients with liver-kidney yin deficiency pattern,and the increased abundance of Patescibacteria and Clostridium may be the characteristic of POI patients with liver-kidney yin deficiency pattern.The decreased of the abundance of Lactobacillus and Bacteroides may be the characteristic of POI patients with spleen and kidney yang deficiency pattern.