一种萘碳酰腙荧光探针对Al3+检测和生物成像研究OA

Two naphtho-carbonyl hydantoin-based fluorescent probes L1 and L2 were designed and synthesized,and their structures were characterized by proton nuclear magnetic resonance(1HNMR)spectroscopy and electrospray ionization mass spectrometry(ESI-MS).Among them,probe L1(10 μmol/L)exhibited high selectivity and sensitivity toward Al3+in an aqueous medium composed of V(ethanol)∶V(HEPES buffer)=3∶7(pH 7.4),with negligible interference from other metal ions.The binding mechanism was investigated using Job's Pot analysis,potentiometric titration,ESI-MS,1HNMR spectroscopy,and density functional theory(DFT)calculations.The results indicated that the sensing response arose from inhibition of photoinduced electron transfer(PET)and restriction of C＝N bond isomerization.DFT calculations suggested an Al(L1)2 binding stoichiometry,which was consistent with the ESI-MS,1 HNMR,and Job's Pot/potentiometric titration results.Fluorescence titration revealed a good linear relationship between the fluorescence intensity of L1 and Al3+concentration within a defined range,with a detection limit as low as 47.3 nmol/L.In addition,probe L1 demonstrated excellent cell membrane permeability and was successfully applied to fluorescence imaging of exogenous Al3+in living cells.Overall,probe L1 functions as a highly selective and sensitive fluorescent probe for Al3+in both aqueous solutions and cellular environments.These findings provide a useful reference for the rational design and development of naphtho-carbonyl hydantoin-based fluorescent probes for metal-ion detection.