双亲性去磷脂填料的制备及其在兽残检测中的应用OA

To address the analytical challenges in veterinary drug residue detection in complex food matrices,particularly the interference from phospholipid that can affect detection accuracy and instrument performance,a novel amphiphilic phospholipid removal material(PPR)was developed.The PPR material was prepared by surface modification of octadecyltrichlorosilane(OTS)on silica gel,providing both hydrophobic and hydrophilic properties.The successful fabrication of the PPR material was confirmed using fourier-transform infrared spectroscopy(FT-IR)for functional group identification,scanning electron microscopy(SEM)for morphological analysis,and nitrogen adsorption-desorption measurements for surface area and pore structure evaluation.A reliable analytical methodology was subsequently established by integrating the PPR filler based solid-phase extraction(SPE)with high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS),enabling rapid purification of phospholipids from food matrices and simultaneous detection of 19 veterinary drug residues.The results showed that the prepared PPR filler had a phospholipid purification rate of 98.58％～99.91％,with a relative standard deviation of 3.9％～5.6％for four food matrices:eggs,whole milk,chicken,and pork.The phospholipid removal rate of PRiME HLB was 94.09％～99.64％,with a relative standard deviation of 2.8％～5.9％;In the animal residue spiked experiments of 19 types of β-receptor agonists and quinolones,the recovery rate of PPR fillers for animal residues in eggs was 81.0％～102.3％,with a relative standard deviation of 1.4％～8.2％.The recovery rate of PRiME HLB for animal residues was 80.5％～104.3％,with a relative standard deviation of 3.1％～9.4％.The phospholipid removal efficiency and residue recovery of PPR fillers are comparable to the performance of commercially available imported products.PPR fillers have the characteristics of simple preparation,good stability,low cost,high phospholipid purification efficiency,indicating potential for widespread application in food safety testing.