转录组学测序鉴定CpG ODN对牙周炎基因疫苗的免疫增强作用OA

Objective:To analyze the potential mechanism by which CpG ODN adjuvants enhance the preventive effect of peri-odontitis gene vaccines at the molecular level by transcriptome sequencing and to screen out key genes and signaling pathways for follow-up research.Methods:A total of nine 4‒6 week-old male Sprague-Dawley rats were randomly divided into three groups.The rats were immunized by intranasal instillation,and euthanize at the 7th week.After total RNA was extracted from the spleen,RNA-seq library construction and sequencing were performed.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Ge-nomes(KEGG)pathway enrichment analysis were used to screen for differentially expressed genes(DEGs).The screened DEGs were transferred to the STRING database to establish a protein-protein interaction(PPI)network map,and the Cytoscape soft-ware was used to further draw the network map.RT-qPCR was used to validate key genes.Results:Based on GO,KEGG,and PPI,five key genes were screened,including Oas2,Irf 7,Np4,Defa11 and Camp.These candidate genes may play a key role in the immune mechanism of periodontitis gene vaccines.The verification of key genes by RT-qPCR showed that the results of the experiment were consistent with the transcriptome sequencing analysis.Conclusion:The results of the experiment are consistent with the transcriptome sequencing analysis of the preventive effect of periodontitis gene vaccines based on transcriptomic sequenc-ing,and screens out five key genes involved in the synergistic effect of CpG ODN adjuvant with periodontitis gene vaccines,which provides a preliminary exploration of the immune mechanism of periodontitis genetic vaccines at the genetic level.