异鼠李素对喉癌细胞生长、迁移及PI3K/AKT1/β-catenin信号的抑制作用OA

Objective:To investigate the effects of isorhamnetin(ISO)on the growth and migration of laryngeal cancer cells and its relationship with PI3K/AKT1/β-catenin signaling.Methods:Laryngeal cancer cell lines(HEP-2,TU212)and human co-lon epithelial cells(NCM460)were used as the research objects,the effects of ISO on the growth and migration of laryngeal can-cer cells were analyzed by MTT assay,colony formation assay,scratch assay,Transwell assay,trypan blue staining,Hoechst 33258 staining,mitochondrial membrane potential detection,and reactive oxygen species detection;Network pharmacology and molecular docking were used to predict the molecular targets of ISO on laryngeal cancer cells;Western blot was used to detect the expressions of p-PI3K,p-AKT1,β-catenin,and cyclin D1 protein in laryngeal cancer cells.Results:Compared to the control group(ISO 0 μmol/L),ISO(10,20,40 μmol/L)significantly inhibited the proliferation,colony formation,migration,and in-vasion of HEP-2 and TU212 cells,and induced the increase of reactive oxygen species level,the decrease of mitochondrial mem-brane potential,and the increase of apoptosis rate.The results of network pharmacology and molecular docking suggested that ISO may have effects on AKT1 molecular targets;Western blot results showed that the protein expression levels of p-PI3K,p-AKT1,β-catenin,and cyclin D1 were significantly lower than those in the control group after HEP-2 and TU212 cells were treated with different concentrations of ISO(10、20、40μmol/L)(P<0.05).Conclusions:ISO can inhibit the growth and migra-tion of laryngeal cancer cells,and induce apoptosis by increasing the level of reactive oxygen species and reducing mitochondrial membrane potential,and its molecular mechanism may be related to the regulation of PI3K/AKT1/β-catenin signaling pathway.