抗菌肽FR-13温敏水凝胶用于皮肤创伤感染的研究OA

Objective:To prepare the antibacterial peptide FR-13 loaded with chitosan thermosensitive hydrogel(FR-13/CS),and to investigate its physicochemical properties,antibacterial activity,and wound healing effects in mice,for providing a new strategy in treating skin injuries caused by battle wounds.Methods:The FR-13/CS hydrogel was prepared using a physical cross-linking method,and its physicochemical properties were characterized by a scanning electron microscopy,rheology,mechanical testing,and in vitro degradation testing.Additionally,the biocompatibility and antibacterial effects of the FR-13/CS hydrogel were evaluated.By using a full-thickness skin wound model in mice,its effect on promoting wound healing was investigated.Re-sults:The FR-13/CS hydrogel exhibited excellent thermosensitive properties,a uniform microstructure,suitable mechanical strength,and good biodegradability.The results of in vitro antibacterial tests demonstrated that FR-13/CS exhibited significant an-tibacterial activity against Escherichia coli and Staphylococcus aureus.In vivo pharmacodynamic experiments showed that the FR-13/CS hydrogel significantly promoted wound healing in mice.Safety assessments confirmed that FR-13/CS had good bio-compatibility.Conclusion:The FR-13/CS hydrogel possesses antibacterial activity and promotes wound healing,demonstrating a promising potential for clinical applications.