子宫内膜癌组织CCNA2、CDCA8表达与增殖侵袭基因、临床病理参数及预后的关系研究OA

Objective To investigate relationships of expressions of cyclin A2(CCNA2)and cell division cycle-related gene 8(CDCA8)in endometrial carcinoma(EC)tissues with expressions of proliferation genes and invasion genes,clinicopathological parameters and prognosis of the disease.Methods 203 EC patients who underwent surgical treatment in The First Affiliated Hospital of Hebei North University from January 2016 to February 2020 were selected as study subjects.Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)was employed to detect expressions of CCNA2,CDCA8 and proliferation genes[cyclin-dependent kinase 6(CDK6),proliferating cell nuclear antigen(PCNA),cyclin D1(CCND1),ribonucleotide reductase M2(RRM2)],invasion genes[high mobility group protein B1(HMGB1),Dickkopf-related protein 1(DKK1),metastasis-associated protein 1(MTA1)]in EC tissues and adjacent tissues of EC patients.Pearson correlation analysis was used to analyzed correlations of expressions of CCNA2 and CDCA8 with expressions of CDK6,PCNA,CCND1,RRM2,HMGB1,DKK1 and MTA,and relationships of expressions of CCNA2 and CDCA8 in EC tissues with clinicopathological parameters of the patients.Kaplan-Meier survival curve was used to analyze effects of high expressions and low expressions of CCNA2 and CDCA8 on prognosis(overall 3-year survive rate)of EC patients.Results The relative mRNA expression levels of CCNA2,CDCA8,CDK6,PCNA,CCND1,RRM2,HMGB1 and MTA1 in EC tissues were higher than those in the adjacent tissues,the differences were statistically significant(t=11.986-30.927,all P<0.05),while the relative expression of DKK1 mRNA in EC tissues was lower than that in the adjacent tissues,and the difference was statistically significant(t=17.033,P<0.05).The relative mRNA expressions of CCNA2 and CDCA8 in EC tissues were positively correlated with the relative mRNA expressions of CDK6,PCNA,CCND1,RRM2,HMGB1 and MTA1(r=0.538-0.665,all P<0.05),while they were negatively correlated with the relative mRNA expression of DKK1(r=-0.593 and-0.606 respectively,both P<0.05).The relative expression levels of CCNA2 mRNA and CDCA8 mRNA in EC tissues were related to clinicopathological parameters such as International Federation of Gynecology and Obstetrics(FIGO)staging,degree of differentiation,depth of myometrial invasion and lymph node metastasis:in detail,the relative expression levels of CCNA2 mRNA and CDCA8 mRNA in such EC tissues groups as FIGO stage III subgroup,low differentiation group,depth of myometrial invasion≥1/2 group and lymph node metastasis group were higher,and the differences were statistically significant(t=6.948-13.894,all P<0.05).The overall 3-year survival rate of EC patients in high CCNA2 expression group was 72.34%,which was lower than 91.43%in low CCNA2 expression group,the difference was statistically significant(χ2=12.405,P<0.001).The overall 3-year survival rate of EC patients in high CDCA8 expression group was 71.43%,which was lower than 93.07%in low CDCA8 expression group,the difference was statistically significant(χ2=15.990,P<0.001).Conclusion CCNA2 and CDCA8 are highly expressed in EC tissues,and their expression levels are related to proliferation genes and invasion genes,and clinicopathological parameters of the patients.Elevated expression levels of CCNA2 and CDCA8 can lead to poor prognosis of EC patients.