哮喘患儿miR-185-5p、CDC42表达水平与病情严重程度的关系OA
Relationship between miR-185-5p and CDC42 expression levels and disease severity in children with asthma
目的 探索哮喘患儿血清微小RNA-185-5p(miR-185-5p)、细胞分裂周期蛋白42(CDC42)的表达及与疾病严重程度的关系.方法 研究选取了2022年1月至2024年1月在河北北方学院附属第一医院住院的105例哮喘患儿及80例健康对照儿童,根据病情严重程度将哮喘患儿分为轻中度组70例和重度组35例.比较三组儿童血清miR-185-5p及CDC42水平;采用Pearson相关分析血清 miR-185-5p、CDC42水平与免疫球蛋白E(IgE)、干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)的相关性;采用Logistic回归分析影响重症哮喘的因素;利用受试者工作特征(ROC)曲线分析血清miR-185-5p、CDC42水平对重症哮喘的诊断价值.结果 对照组、轻中度组、重度组哮喘患儿miR-185-5p、CDC42水平均依次增高,差异有统计学意义(F值分别为241.335、95.161,P<0.05);轻中度组IgE及IL-4低于重度组,而IFN-γ高于重度组,差异有统计学意义(t值分别为4.179、4.585、5.205,P<0.05);哮喘患儿miR-185-5p、CDC42水平与IgE、IL-4均呈正相关关系(r值分别为0.490、0.479、0.541、0.361,P<0.05),与IFN-γ均呈负相关关系(r值分别为-0.358、-0.410,P<0.05);miR-185-5p与CDC42表达呈正相关性且有靶向结合位点(r=0.512,P<0.001);多因素Logistic回归分析结果提示 miR-185-5p、CDC42是哮喘患儿出现重症的危险因素,IFN-γ是其保护因素(OR值分别为1.345、1.061、0.006,P<0.05);血清miR-185-5p与CDC42水平单独及联合诊断重症哮喘的曲线下面积(AUC)分别为0.793、0.885、0.898.结论 支气管哮喘患儿血清miR-185-5p及CDC42水平显著升高,且与患儿病情严重程度密切相关,两者联合检测对患儿支气管哮喘严重程度具有较高预测价值.
Objective To investigate the expression levels of serum microRNA-185-5p(miR-185-5p)and cell division cycle protein 42(CDC42)in children with asthma and to explore their relationships with disease severity.Methods A total of 105 children with asthma hospitalized at the First Affiliated Hospital of Hebei North University from January 2022 to January 2024 were enrolled,along with 80 healthy children as controls.According to disease severity,asthmatic children were divided into a mild-to-moderate group(n=70)and a severe group(n=35).Serum levels of miR-185-5p and CDC42 were compared among the three groups.Pearson correlation analysis was used to evaluate the correlations between serum miR-185-5p and CDC42 levels and immunoglobulin E(IgE),interferon-γ(IFN-γ),and interleukin-4(IL-4).Logistic regression analysis was performed to identify factors influencing severe asthma.Receiver operator characteristic(ROC)curves were used to assess the diagnostic value of serum miR-185-5p and CDC42 levels for severe asthma.Results Serum miR-185-5p and CDC42 levels increased sequentially in the control group,mild-to-moderate group,and severe group,with statistically significant differences(F=241.335 and 95.161,respectively,P<0.05).Compared with the severe group,the mild-to-moderate group had significantly lower levels of IgE and IL-4 and higher levels of IFN-γ(t=4.179,4.585,and 5.205,respectively,P<0.05).Serum miR-185-5p and CDC42 levels were positively correlated with IgE and IL-4(r=0.490,0.479,0.541,and 0.361,respectively,P<0.05)and negatively correlated with IFN-γ(r=-0.358 and-0.410,respectively,P<0.05).A positive correlation was observed between miR-185-5p and CDC42 expression,and a potential targeting relationship was identified(r=0.512,P<0.001).Multivariate logistic regression analysis indicated that miR-185-5p and CDC42 were risk factors for severe asthma,whereas IFN-γ was a protective factor(OR=1.345,1.061,and 0.006,respectively,P<0.05).The area under the curve(AUC)values for serum miR-185-5p and CDC42 in diagnosing severe asthma were 0.793 and 0.885,respectively,and the combined AUC reached 0.898.Conclusion Serum miR-185-5p and CDC42 levels are significantly elevated in children with bronchial asthma and are closely associated with disease severity.Combined detection of miR-185-5p and CDC42 has high predictive value for assessing asthma severity in children.
李春梅;李婷婷;马俊帅;宋艳红;张艳文;卢艳辉;刘振奎;郭卫平;李文斌
河北北方学院附属第一医院儿内科,河北 张家口 075000张家口市妇幼保健院儿科,河北 张家口 075000河北北方学院附属第一医院检验科,河北 张家口 075000河北北方学院附属第一医院儿内科,河北 张家口 075000河北北方学院附属第一医院儿内科,河北 张家口 075000河北北方学院附属第一医院儿内科,河北 张家口 075000河北北方学院附属第一医院儿内科,河北 张家口 075000河北北方学院附属第一医院儿内科,河北 张家口 075000河北北方学院附属第一医院儿内科,河北 张家口 075000
医药卫生
儿童哮喘微小RNA-185-5p细胞分裂周期蛋白42病情程度
childasthmamicroRNA-185-5pcell division cycle protein 42disease severity
《中国妇幼健康研究》 2026 (3)
16-21,6
河北省卫生健康委医学科学研究课题(20231436)
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