苦瓜几丁质酶基因家族鉴定及其在枯萎病菌侵染后的表达分析OA
Identification and Expression Analysis of Chitinase Gene Family in Momordica charantia after Fusarium oxysporum Infection
[目的]植物几丁质酶(Chitinase,CHi)是一种病程相关蛋白,在植物防御真菌过程中发挥关键作用.该研究旨在系统解析苦瓜CHi基因家族,并揭示其在响应枯萎病菌侵染时的表达变化.[方法]以拟南芥CHi基因的蛋白序列为查询序列,在苦瓜基因组数据库中进行BLASTp比对,鉴定出苦瓜CHi基因家族成员,并开展理化性质、系统发育树、基因结构分析等.通过对苗期苦瓜根部人工接种尖孢镰刀菌 3 d,RNA-seq分析苦瓜根部组织CHi基因表达情况.[结果]全基因组共鉴定出 38 个苦瓜CHi基因家族成员,分别位于 1、3、4、5、6、8、10号染色体上.理化分析结果显示,苦瓜CHi基因家族成员编码 233～879 个氨基酸,分子量为 25.83～95.43 kDa,等电点分布在 4.62～9.78,不稳定系数为 22.25～96.54,亲水性为-0.893～0.104.根据系统进化树分析,这些基因被划分为 5 类,同一类成员间的保守结构域具有较高相似性.启动子区域作用元件分析结果显示,TATA-box和CAAT-box存在于所有CHi基因中,且显著多于其他顺式作用元件.接种尖孢镰刀菌 3 d后,抗病材料'K49'中呈现上调表达的CHi基因数量显著多于感病材料'CP92',其中除GH19 亚家族中的McCHi1 外,其余 13 个显著上调表达的基因均属于GH18 亚家族.[结论]该研究首次完成苦瓜CHi基因家族的系统鉴定及响应枯萎病菌侵染过程中基因家族成员的表达特征,上调表达的CHi基因在苦瓜抗枯萎病防御中可能起关键作用,为后续抗病机制解析与育种应用提供重要依据.
[Objective]Plant chitinase(CHi)is a pathogenesis-related protein that plays a crucial role in plant defense against fungi.This study aims to systematically characterize the CHi gene family in Momordica charantia and reveal its expression changes in response to Fusarium oxysporum infection.[Method]Using Arabidopsis thaliana chitinase protein sequences as the query sequence,BLASTp alignment was performed in M.charantia genome.Identified CHi genes were characterized in terms of physicochemical properties,phylogenetic analysis,and gene structures.Additionally,RNA-seq analysis was conducted on root tissues of seedlings inoculated with F.oxysporum for 3 d to examine the expression profiles of CHi genes.[Result]A total of 38 M.charantia CHi genes were identified,located on chromosomes 1,3,4,5,6,8,and 10,respectively.Physicochemical analysis revealed that the members of the CHi gene family encode 233 to 879 aa,with molecular weights ranging from 25.83 to 95.43 kDa,isoelectric points between 4.62 and 9.78,instability indices from 22.25 to 96.54,and grand average of hydropathicity(GRAVY)from-0.893 to 0.104.Phylogenetic analysis classified them into five groups,with members within the same group showing high similarity in conserved domains.Analysis of the promoter region showed that TATA-box and CAAT-box elements were present in all CHi genes and were significantly more abundant than other cis-acting elements.Furthermore,the number of CHi genes showing up-regulated expression in the resistant cultivar'K49'was significantly higher than that in the susceptible cultivar'CP92'at three days after inoculation with F.oxysporum.Among these up-regulated genes,except for McCHi1 from the GH19 subfamily,the remaining 13 significantly upregulated genes all belonged to the GH18 subfamily.[Conclusion]This study provides the first systematic identification and expression analysis of the CHi gene family in M.charantia under F.oxysporum infection.The findings indicate that up-regulated CHi genes are likely play a key role in the defense response against fusarium wilt,offering valuable insights for further research on disease resistance mechanisms and breeding applications.
刘小茜;彭成盛;何明;龚浩;郑晓明;邓丽婷;李俊星;赵钢军;冯诚诚
广东省农业科学院蔬菜研究所/广东省蔬菜新技术研究重点实验室,广东 广州 510640广东省农业科学院蔬菜研究所/广东省蔬菜新技术研究重点实验室,广东 广州 510640广东省农业科学院蔬菜研究所/广东省蔬菜新技术研究重点实验室,广东 广州 510640广东省农业科学院蔬菜研究所/广东省蔬菜新技术研究重点实验室,广东 广州 510640广东省农业科学院蔬菜研究所/广东省蔬菜新技术研究重点实验室,广东 广州 510640广东省农业科学院蔬菜研究所/广东省蔬菜新技术研究重点实验室,广东 广州 510640广东省农业科学院蔬菜研究所/广东省蔬菜新技术研究重点实验室,广东 广州 510640广东省农业科学院蔬菜研究所/广东省蔬菜新技术研究重点实验室,广东 广州 510640广西壮族自治区农业科学院蔬菜研究所,广西 南宁 530007
农业科技
苦瓜几丁质酶枯萎病基因家族系统发育树分析基因结构表达分析
Momordica charantiachitinasefusarium wiltgene familyphylogenetic tree analysisgene structureexpression analysis
《广东农业科学》 2026 (1)
1-12,12
广西科技重大专项(桂科AA23023001)广东省农业科学院"金颖人才"项目(R2026PY-TJ008)
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