基于RNA-seq分析粉葛与野葛淀粉积累相关基因的差异表达特征OA

[Objective]This study aimed to investigate the key factors underlying the differential starch accumulation between Pueraria montana var.thomsonii(P.thomsonii)and Pueraria montana var.lobata(P.lobata),to provide a theoretical basis for the molecular breeding of high-starch kudzu varieties.[Method]Six medicinal and edible kudzu resources were collected.The morphological and starch granule characteristics of one-year-old roots were analyzed.Differential transcriptome analysis was performed to identify differentially expressed genes(DEGs),and correlation analysis was conducted to pinpoint genes associated with starch accumulation.[Result]The P.thomsonii accession G1 exhibited the highest starch content,which was twice that of P.lobata,and demonstrated more rapid development and expansion of secondary structural tissue cells.Transcriptome analysis revealed 4718 DEGs,predominantly enriched in functional categories including biological regulation,cellular membrane structures,extracellular regions,plasmodesmata,transcription factors,and signal transduction.Furthermore,137 and 231 genes were significantly positively and negatively correlated with starch accumulation,respectively.Among these,nine genes involved in starch and sugar metabolism and twelve transcription factors were identified as potential key regulators of starch accumulation specificity.[Conclusion]This study establishes a comparative transcriptome database and identifies critical gene set influencing starch accumulation,thereby providing a solid theoretical foundation for the transcriptional regulation and molecular breeding of P.thomsonii.